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Titolo:
The YXXL sequences of a transmembrane protein of bovine leukemia virus arerequired for viral entry and incorporation of viral envelope protein into virions
Autore:
Inabe, K; Nishizawa, M; Tajima, S; Ikuta, K; Aida, Y;
Indirizzi:
RIKEN,Japan Phys & Chem Res, Tsukuba Life Sci Ctr, Ibaraki, Osaka 3050074,RIKEN Ibaraki Osaka Japan 3050074 a Life Sci Ctr, Ibaraki, Osaka 3050074, Hokkaido0815,, Inst Immunol Sci, Sect Serol, Kita Ku, Sapporo, Hokkaido 060 Hokkaido Univ Sapporo Hokkaido Japan 0600815 ta Ku, Sapporo, Hokkaido 060
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 2, volume: 73, anno: 1999,
pagine: 1293 - 1301
SICI:
0022-538X(199902)73:2<1293:TYSOAT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMIAN IMMUNODEFICIENCY VIRUS; PFIZER MONKEY VIRUS; CYTOPLASMIC DOMAIN; SIGNAL-TRANSDUCTION; MEMBRANE-PROTEIN; INTRACELLULAR-TRANSPORT; OLIGOMERIC STRUCTURE; ACTIVATION MOTIF; ANTIGEN RECEPTOR; MATRIX PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Aida, Y RIKEN,Osaka Phys & Chem Res, Tsukuba Life Sci Ctr, 3-1-1 Koyadai, Ibaraki, RIKEN 3-1-1 Koyadai Ibaraki Osaka Japan 3050074 Koyadai, Ibaraki,
Citazione:
K. Inabe et al., "The YXXL sequences of a transmembrane protein of bovine leukemia virus arerequired for viral entry and incorporation of viral envelope protein into virions", J VIROLOGY, 73(2), 1999, pp. 1293-1301

Abstract

The cytoplasmic domain of an envelope transmembrane glycoprotein (gp30) ofbovine leukemia virus (BLV) has two overlapping copies of the (YXXL)(2) motif. The N-terminal motif has been implicated in in vitro signal transduction pathways from the external to the intracellular compartment and is also involved in infection and maintenance of high viral loads in sheep that have been experimentally infected,vith BLV. To determine the role of YXXL sequences in the replication of BLV in vitro, we changed the tyrosine or leucine residues of the N-terminal motif in an infectious molecular clone of BLV,pBLV-IF, to alanine to produce mutated proviruses designated Y487A, L490A,Y498A, L501A, and Y487/498A. Transient transfection of African green monkey kidney COS-1 cells with proviral DNAs that encoded wild-type and mutant sequences revealed that all of the mutated proviral DNAs synthesized mature envelope proteins and released virus particles into the growth medium. However, serial passages of fetal lamb kidney (FLK) cells, which are sensitive to infection with BLV, after transient transfection revealed that mutation of a second tyrosine residue in the N-terminal motif completely prevented the propagation of the virus. Similarly, Y498A and Y487/498A mutant BLV thatwas produced by the stably transfected COS-1 cells exhibited significantlyreduced levels of cell-free virion-mediated transmission. Analysis of the protein compositions of mutant viruses demonstrated that lower levels of envelope protein were incorporated by two of the mutant virions than by wild-type and other mutant virions. Furthermore, a mutation of a second tyrosineresidue decreased the specific binding of BLV particles to FLK cells and the capacity for viral penetration. Our data indicate that the YXXL sequences play critical roles in both viral entry and the incorporation of viral envelope protein into the virion during the life cycle of BLV.

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Documento generato il 22/01/20 alle ore 09:50:46