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Titolo:
Chimeric 7E3 Fab (ReoPro) decreases detectable CD11b on neutrophils from patients undergoing coronary angioplasty
Autore:
Mickelson, JK; Ali, MN; Kleiman, NS; Lakkis, NM; Chow, TW; Hughes, BJ; Smith, CW;
Indirizzi:
Baylor Coll Med, Dept Med, Cardiol Sect, Houston, TX 77030 USA Baylor CollMed Houston TX USA 77030 Cardiol Sect, Houston, TX 77030 USA BaylorTXoll Med, Dept Pediat, Speros P Martel Lab Leukocyte Biol, Houston,Baylor Coll Med Houston TX USA 77030 Martel Lab Leukocyte Biol, Houston, Vet Adm Med Ctr, Houston, TX 77211 USA Vet Adm Med Ctr Houston TX USA 77211 t Adm Med Ctr, Houston, TX 77211 USA Rice Univ, Houston, TX 77030 USA Rice Univ Houston TX USA 77030Rice Univ, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
fascicolo: 1, volume: 33, anno: 1999,
pagine: 97 - 106
SICI:
0735-1097(199901)33:1<97:C7F(DD>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE-PLATELET ADHESION; CARDIOPULMONARY BYPASS; UNSTABLE ANGINA; P-SELECTIN; MYOCARDIAL-INFARCTION; BALLOON ANGIOPLASTY; CHEMOTACTIC FACTORS; STENT IMPLANTATION; SURFACE EXPRESSION; ARTERY DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, CW Childrens Nutr Res Ctr, Room 6014,1100 Bates, Houston, TX 77030 USA Childrens Nutr Res Ctr Room 6014,1100 Bates Houston TX USA 77030
Citazione:
J.K. Mickelson et al., "Chimeric 7E3 Fab (ReoPro) decreases detectable CD11b on neutrophils from patients undergoing coronary angioplasty", J AM COL C, 33(1), 1999, pp. 97-106

Abstract

Objectives. The purpose of this study was to monitor the effects of chimeric 7E3 Fab (ReoPro) on leukocyte and platelet activation and interaction during coronary angioplasty. Background. Increased expression of CD11b on monocytes and neutrophils promotes their adhesion to endothelial cells, extracellular matrix and smooth muscle cells. Thrombin-activated platelets adhere via P-selectin to monocytes and neutrophils. These cell interactions mag affect the outcome of coronary angioplasty. Methods. During coronary angioplasty, venous blood was obtained for flow cytometric detection of leukocyte CD11b; platelet CD41a, CD61a and CD62P; the percentage of leukocytes with adherent platelets and the intensity of bound platelet fluorescence. Results. Leukocyte CD11b expression increased after angioplasty in controlpatients (neutrophils 171 +/- 25 to 255 +/- 31 mean fluorescence intensity[MFI, mean ct SEM], n = 25, p < 0.0001; monoecytes 200 +/- 40 to 248 +/- 36 MFI, n = 17, p < 0.05) and decreased in the patients selected to receive chimeric 7E3 Fab (neutrophils 146 +/- 30 to 82 +/- 22 MFI, n = 25, p < 0.0001; monocytes 256 +/- 53 to 160 +/- 38 MFI, n 17, p < 0.05). Neutrophil CD11b decreased after in vitro incubation of whole blood with chimeric 7E3 Fab(n = 5, p = 0.01), but fMLP-induced increases in CD11b were not prevented. The CD11b expression was unchanged and increased with fMLP stimulation after in vitro incubation of isolated neutrophils with chimeric 7E3 Fab. Direct-labeled chimeric 7E3 Fab was not detected bound to neutrophils in whole blood or isolated cells using bow cytometric techniques. Adhesion of isolated neutrophils to protein coated glass was not prevented by in vitro incubation with chimeric 7E3 Fab. Platelet activation increased after angioplasty in control patients (CD62P 8.9 +/- 0.8 to 12.3 +/- 1.2 MFI, n = 25, p < 0.05; CD41a 382 +/- 25 to 454 +/- 26 MFI, n = 25, p < 0.05, CD61a 436 +/- 52 to 529 +/- 58 MFI, n = 11, p < 0.05); it did not increase in the patients selected to receive chimeric 7E3 Fab (CD62P 13.2 +/- 1.0 to 9.0 +/- 0.9 MFI, n = 25, p < 0.05; CD61a 398 +/- 32 to 410 +/- 38 MFI, n = 7, p = NS). Leukocytes with adherent platelets tended to increase in the control group of patients and decrease after the procedure in patients selected to receive chimeric 7E3 Fab; individual and procedure-related variability were marked. Conclusions. Despite standard aspirin and heparin therapy, leukocyte and platelet activation with platelet adherence to leukocytes occurs after coronary angioplasty. Although chimeric 7E3 Fab does not bind to leukocytes directly, it influences CD11b expression in whole blood. Modulation of plateletand leukocyte activation and interaction by chimeric 7E3 Fab may contribute to an improved outcome after coronary angioplasty. (C) 1998 by the American College of Cardiology.

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Documento generato il 23/01/21 alle ore 10:10:27