Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Electrochemical monitoring of biogenic amine neurotransmission in real time
Autore:
Michael, DJ; Wightman, RM;
Indirizzi:
Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 Chem, Chapel Hill, NC 27599 USA Univ N Carolina, Curriculum Neurobiol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 obiol, Chapel Hill, NC 27599 USA
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 1-2, volume: 19, anno: 1999,
pagine: 33 - 46
SICI:
0731-7085(199902)19:1-2<33:EMOBAN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL-MEDULLARY CELLS; FAST CYCLIC VOLTAMMETRY; EVOKED EXTRACELLULAR DOPAMINE; INDIVIDUAL CHROMAFFIN CELLS; RAT-BRAIN SLICES; IN-VIVO; CATECHOLAMINE RELEASE; INVIVO VOLTAMMETRY; NUCLEUS-ACCUMBENS; VESICLE FUSION;
Keywords:
fast-scan cyclic voltammetry; carbon fiber microelectrodes; neurotransmitters; biogenic amines; amperometry; high speed chronoamperometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Wightman, RM Univ N Carolina, Dept Chem, CB 3290 Venable Hall, Chapel Hill, NC 27599 USA Univ N Carolina CB 3290 Venable Hall Chapel Hill NC USA 27599
Citazione:
D.J. Michael e R.M. Wightman, "Electrochemical monitoring of biogenic amine neurotransmission in real time", J PHARM B, 19(1-2), 1999, pp. 33-46

Abstract

Three techniques, constant-potential amperometry, high-speed chronoamperometry, and fast-scan cyclic voltammetry, have been used extensively to investigate the rapid events associated with neurotransmission. These techniquesvary in sensitivity, chemical resolution and temporal resolution. Amperometry provides the best temporal resolution but little chemical resolution. Fast-scan cyclic voltammetry provides both good temporal and chemical resolution, while high-speed chronoamperometry offers good temporal resolution and moderate chemical resolution. The amount of chemical information which isneeded for a neurochemical measurement depends upon the sample. For singlecells, secondary methods, such as HPLC and capillary electrophoresis, offer extensive chemical information about the contents of a cell. With this information, chemical information is not needed during the electrochemical measurement. Therefore, amperometry is employed to obtain the greatest temporal resolution. However, when using more complex biological samples, such asbrain slices or in vivo implantation, there is a greater demand for chemical resolution provided by the electrochemical measurement. To bolster results, further confirmation is sought from anatomical, physiological and pharmaceutical evidence. Within this review, the three electrochemical techniques are outlined and compared. Examples are then provided of measurements which have been made in the three predominant biological samples which have been studied: single cells, brain slices and intact animals. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 21:55:35