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Titolo:
Intravenous cannulation of the freely behaving rat: frequent blood sampling and volume-dependent effect on blood leukocyte counts
Autore:
Nave, H; von Horsten, S; Helfritz, F; Kuhlmann, S; Ballof, J; Drube, J; Pabst, R;
Indirizzi:
Hannover Med Sch, Dept Funct & Appl Anat, D-30625 Hannover, Germany Hannover Med Sch Hannover Germany D-30625 nat, D-30625 Hannover, Germany
Titolo Testata:
JOURNAL OF EXPERIMENTAL ANIMAL SCIENCE
fascicolo: 3, volume: 39, anno: 1998,
pagine: 67 - 77
SICI:
0939-8600(199812)39:3<67:ICOTFB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SECRETION; INFUSION; STRESS;
Keywords:
blood sampling; venous cannulation; volume stress; leukocytosis; freely moving rat; hypervolemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Nave, H Hannover25ed Sch, Dept Funct & Appl Anat, OE 4120,Carl Neuberg Str1, D-306 Hannover Med Sch OE 4120,Carl Neuberg Str 1 Hannover Germany D-30625
Citazione:
H. Nave et al., "Intravenous cannulation of the freely behaving rat: frequent blood sampling and volume-dependent effect on blood leukocyte counts", J EXP ANIM, 39(3), 1998, pp. 67-77

Abstract

A procedure for chronic cannulation of the external jugular vein of freelymoving adult rats is described and compared to other techniques. This method permits multiple daily collections of blood and application of drugs for22 days +/- 1.2 SEM (n = 50). Animals can be used for various experiments and they do not need to be restrained or anesthetized for blood sampling. Adistinct improvement compared to other methodologies is the combination offixation, suspension of the catheter and the kind of anticoagulation. The advantages of this method are that the number of animals needed for experimentation can be reduced, as well as the long duration of catheter patency, low cost and minimal postoperative care. Furthermore, by using this technique, a new phenomenon of hypervolemia-induced leucocytosis is described. While a single injection of 350 mu l/min saline has no effect on leukocyte numbers, a single injection of 750 mu l/min saline induces an early rapid leukocytosis of 160% within 1 minute and a late second peak after 1 hour of 180%. In conclusion, this blood sampling technique makes it possible to study dynamic alterations in the blood of completely unstressed animals. However,one limitation in its use is that blood leukocyte numbers are affected at an injected volume of >350 mu l.

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Documento generato il 09/04/20 alle ore 07:12:40