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Titolo:
The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow
Autore:
Robinson, RS; Mann, GE; Lamming, GE; Wathes, DC;
Indirizzi:
Univ London Royal Vet Coll, Dept Vet Basic Sci, Potters Bar EN6 1NB, Herts, Univ London Royal Vet Coll Potters Bar Herts England EN6 1NB 1NB, Herts, Univcs,ttingham, Sch Biol Sci, Div Anim Physiol, Loughborough LE12 5RD, Lei Univ Nottingham Loughborough Leics England LE12 5RD borough LE12 5RD, Lei
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 1, volume: 160, anno: 1999,
pagine: 21 - 33
SICI:
0022-0795(199901)160:1<21:TEOPOT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROUS-CYCLE; GENE-EXPRESSION; OVARIECTOMIZED EWES; BOVINE ENDOMETRIUM; OVINE UTERUS; ESTRADIOL; SHEEP; LOCALIZATION; HORMONE; PROSTAGLANDIN-F2-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Wathes, DC UnivN6ondon Royal Vet Coll, Dept Vet Basic Sci, Hawkshead Rd, Potters Bar E Univ London Royal Vet Coll Hawkshead Rd Potters Bar Herts England EN6 1NB
Citazione:
R.S. Robinson et al., "The effect of pregnancy on the expression of uterine oxytocin, oestrogen and progesterone receptors during early pregnancy in the cow", J ENDOCR, 160(1), 1999, pp. 21-33

Abstract

The expression of oxytocin receptor (OTR) in the uterine endometrium playsan important role in the initiation of luteolysis. During early pregnancy,the conceptus secretes interferon tau (IFN tau) which inhibits OTR up-regulation and luteolysis. In this study, uterine horn cross sections were collected on day 16 from 15 pregnant cows (PREG), 9 uninseminated controls and 5 inseminated cows with no embryo present. The latter two groups had similar results and were combined to form a single non-pregnant (NP) group. The animals were given an oxytocin challenge shortly before tissue collection toassess prostaglandin F-2 alpha (PGF(2 alpha)) release through the measurement of the metabolite 13,14-dihydro-15-keto PGF(2 alpha) (PGFM). The mRNAs for OTR, oestrogen receptor (ER) and progesterone receptor (PR) were localised by in situ hybridisation. The results were quantified by optical density (OD) measurements h-om autoradiographs using image analysis. OTR protein was measured by autoradiography with iodinated oxytocin antagonist and ER and PR protein was detected by immunocytochemistry. The release of PGFM after the oxytocin challenge was significantly higher in the 14 NP cows (187% +/- 15%) compared with the PREG group (131% +/- 11%) (P < 0.01). Low concentrations of OTR mRNA were localised to the luminal epithelium (LE) in 6 out of the 14 NP cows, of which 2 also expressed OTR protein, while OTR mRNA and protein were undetectable in all the pregnant animals. These results indicated that the sampling time coincided with the onset of the luteolytic mechanism in the NP cows. On day 16 ER mRNA was detectable in both the LE and glands of both PREG and NP animals. There were no differences in either ER mRNA or protein between NP and PREG samples. PR mRNA was moderately expressed in the caruncular stroma, with lower levels in the dense caruncular-likestroma and glands. There were no differences between PREG and NP animals. The expression of PR mRNA and protein in the deep glands was variable between animals. These results suggested that, in cows, the presence of an embryo suppressed the expression of OTR, but had no effect on the expression of the transcriptionally regulated ER on day 16.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:30:54