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Titolo:
Proabsorptive and prosecretory roles for nitric oxide in cholera toxin induced secretion
Autore:
Turvill, JL; Mourad, FH; Farthing, MJG;
Indirizzi:
St1Bartholomews & Royal London Sch Med & Dent, Digest Dis Res Ctr, London E St Bartholomews & Royal London Sch Med & Dent London England E1 2AD on E
Titolo Testata:
GUT
fascicolo: 1, volume: 44, anno: 1999,
pagine: 33 - 39
SICI:
0017-5749(199901)44:1<33:PAPRFN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL ELECTROLYTE TRANSPORT; ORAL REHYDRATION SOLUTION; JEJUNUM IN-VIVO; L-ARGININE; RAT ILEUM; DIARRHEAL DISEASE; FLUID SECRETION; 5-HYDROXYTRYPTAMINE; ABSORPTION; INHIBITION;
Keywords:
cholera toxin; nitric oxide; small intestinal transport; 5-hydroxytryptamine; L-arginine; nitric oxide synthase inhibitors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Turvill, JL Stt,artholomews & Royal London Sch Med & Dent, Digest Dis Res Ctr, Turner S St Bartholomews & Royal London Sch Med & Dent Turner St London England E1 2AD
Citazione:
J.L. Turvill et al., "Proabsorptive and prosecretory roles for nitric oxide in cholera toxin induced secretion", GUT, 44(1), 1999, pp. 33-39

Abstract

Background-Cholera toxin causes small intestinal hypersecretion by inducing a coordinated response from enterocytes, enterochromaffin cells, enteric neurones, and the vascular supply. Nitric oxide has been implicated in the function of these separate components. Aims-To explore the role of nitric oxide in the totality of cholera toxin induced secretion in vivo. Methods-One group of adult male Wistar rats was treated with the nitric oxide synthase inhibitors N-G-nitro-L-arginine methyl ester (L-NAME; subcutaneously or intraluminally), N-G-methyl-L-arginine (L-NMA), or 7-nitroindazole. A second group of rats was treated with L-arginine (intraperitoneally orintraluminally) or D-arginine. The small intestine was isolated between two cannulae and instilled with 75 mu g cholera toxin or saline for two hours. Small intestinal perfusion of a plasma electrolyte solution containing [C-14]-PEG was undertaken to determine net water and electrolyte movement. After the experiment macroscopic and microscopic intestinal appearances were noted and jejunal 5-hydroxytryptamine concentrations were determined. Results-Both L-arginine and L-NAME induced secretion in the basal state, but only when administered intraluminally. Systemically applied L-NAME caused a dose dependent reduction in cholera toxin induced secretion. This was paralleled by L-NMA but not by 7-nitroindazole or by intraluminally applied L-NAME. Systemically applied L-NAME caused notable cyanosis of the intestine, consistent with mesenteric ischaemia, but no microscopic abnormalities. Systemically applied L-arginine but not D-arginine also reduced cholera toxin induced secretion and inhibited 5-hydroxytryptamine release. Conclusion-Nitric oxide has a duality of roles in cholera toxin induced secretion, acting both as an absorbagogue and a acting both as an absorbagogue ana a Secretagogue. Its mechanisms of action include the maintenance of mucosal perfusion and enterochromaffin cell stabilisation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:20:34