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Titolo:
A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector
Autore:
Mousli, M; Devaux, C; Rochat, H; Goyffon, M; Billiald, P;
Indirizzi:
Fac Med Nord, Ingn Prot Lab, CNRS, UMR 6560, F-13916 Marseille 20, France Fac Med Nord Marseille France 20 UMR 6560, F-13916 Marseille 20, France Museum Natl Hist Nat, Lerai, F-75231 Paris, France Museum Natl Hist Nat Paris France F-75231 , Lerai, F-75231 Paris, France Fac Pharm, F-37200 Tours, France Fac Pharm Tours France F-37200Fac Pharm, F-37200 Tours, France
Titolo Testata:
FEBS LETTERS
fascicolo: 2-3, volume: 442, anno: 1999,
pagine: 183 - 188
SICI:
0014-5793(19990115)442:2-3<183:ARSAFT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY; ESCHERICHIA-COLI; FV FRAGMENT; PHAGE; AFFINITY; BINDING; SCFV; PROTEINS; LIBRARY; FORMS;
Keywords:
neurotoxin; single-chain antibody fragment; single-chain antibody; neutralization; scorpion; Androctonus australis hector;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Devaux, C Facrseillerd, Ingn Prot Lab, CNRS, UMR 6560, Bvd Pierre Dramard,F-13916 Ma Fac Med Nord Bvd Pierre Dramard Marseille France 20 F-13916 Ma
Citazione:
M. Mousli et al., "A recombinant single-chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector", FEBS LETTER, 442(2-3), 1999, pp. 183-188

Abstract

Monoclonal antibody 4C1 specifically binds to and neutralizes the most potent neurotoxin (AahII) of the scorpion Androctonus nustralis. The cDNAs encoding the variable regions of this antibody were isolated by PCR-mediated cloning. A single-chain Fv gene was engineered and expressed in Escherichia coli, The recombinant protein had neutralizing activity similar to that of the intact antibody in vitro and in vivo. We have thus neutralized the pharmacological and biological properties of a scorpion neurotoxin with a single-chain Fv, which opens nem perspectives for the treatment of envenomizations, (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 09:49:18