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Titolo:
Improved extraction procedure and RIA for determination of arginine(8)-vasopressin in plasma: Role of premeasurement sample treatment and reference values in children
Autore:
Kluge, M; Riedl, S; Erhart-Hofmann, B; Hartmann, J; Waldhauser, F;
Indirizzi:
Univ Vienna, Dept Pediat, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 a, Dept Pediat, A-1090 Vienna, Austria
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 1, volume: 45, anno: 1999,
pagine: 98 - 103
SICI:
0009-9147(199901)45:1<98:IEPARF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARGININE-VASOPRESSIN; RAPID RADIOIMMUNOASSAY; PLATELET VASOPRESSIN; AGGREGATION; ASSAYS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Waldhauser, F Univ Vienna, Dept Pediat, Wahringer Gurtel 18-20, A-1090 Vienna, Austria Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 ia
Citazione:
M. Kluge et al., "Improved extraction procedure and RIA for determination of arginine(8)-vasopressin in plasma: Role of premeasurement sample treatment and reference values in children", CLIN CHEM, 45(1), 1999, pp. 98-103

Abstract

We optimized an RIA for measurement of arginine(8)-vasopressin (AVP) in plasma by use of 100-mg Isolute C-18 columns for extraction, addition of a preincubation step, and use of maximal dilution of a commercially available antiserum. The detection limit was 0.06 ng/L when 0.5 mL of acidified plasmawas extracted. The within- and between-run CVs (n = 16) at physiological concentrations were 5.8-10.2% and 6.5-11.7%, respectively. Prolonged storageof blood at 25 degrees C, but not at 4 degrees C, led to a significant increase in measured plasma AVP concentrations. When plasma samples were prepared at several centrifugation speeds, plasma AVP was significantly correlated with the platelet count (r = 0.899; P <0.001). This emphasizes the need for careful sample preparation to avoid contamination of plasma with platelet-bound AVP. Basal plasma AVP in 203 children and adolescents (105 males and 98 females; ages, 1 day to 18 years) averaged 1.1 +/- 0.6 ng/L. There was no significant difference between males and females and no correlation with age. In 16 healthy adult controls, plasma AVP averaged 1.0 +/- 0.5 ng/L.

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Documento generato il 15/07/20 alle ore 08:22:12