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Titolo:
Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27
Autore:
Mazur, X; Fussenegger, M; Renner, WA; Bailey, JE;
Indirizzi:
ETHrlandch, Inst Biotechnol, Swiss Fed Inst Technol, CH-8093 Zurich, Switze ETH Zurich Zurich Switzerland CH-8093 st Technol, CH-8093 Zurich, Switze
Titolo Testata:
BIOTECHNOLOGY PROGRESS
fascicolo: 5, volume: 14, anno: 1998,
pagine: 705 - 713
SICI:
8756-7938(199809/10)14:5<705:HPOGCH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; TUMOR-SUPPRESSOR GENE; RECOMBINANT CHO CELLS; PROTEIN-FREE MEDIUM; MAMMALIAN-CELLS; CANCER-CELLS; DNA-DAMAGE; NECK-CANCER; FACTOR-BETA; S-PHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Bailey, JE ETHrlandch, Inst Biotechnol, Swiss Fed Inst Technol, CH-8093 Zurich, Switze ETH Zurich Zurich Switzerland CH-8093 CH-8093 Zurich, Switze
Citazione:
X. Mazur et al., "Higher productivity of growth-arrested Chinese hamster ovary cells expressing the cyclin-dependent kinase inhibitor p27", BIOTECH PR, 14(5), 1998, pp. 705-713

Abstract

We constructed stable Chinese hamster ovary (CHO) cell lines which conditionally and coordinately express the model product gene secreted alkaline phosphatase (SEAP) and one of the cytostatic genes p21, p27, and p53175P, a p53 mutant deficient in apoptotic but not cell-cycle arrest function. The use of dicistronic expression technology allowed the conditional expression of the model product gene and the cytostatic gene in a coordinated fashion from a single expression unit under the control of the tetracycline-responsive promoter PhCMV*-1. Due to the presence of a cytostatic gene in the multicistronic expression unit, the growth behavior of the engineered CHO cell lines could be controlled by the addition or withdrawal of the exogenous agent tetracycline to or from the cell culture medium. Withdrawal of tetracycline resulted in sustained growth arrest of the stable cell lines for a prolonged period. The growth arrest of such cell lines was found to be accompanied by a 10-15-fold increase in their production of SEAP per cell. This controlled proliferation technology allows the design of a novel two-stage production process which consists of a proliferation phase leading to the desired cell density, followed by an extended production phase during which thecells remain growth-arrested and increase cell-specific production of a heterologous protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:48:53