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Titolo:
Recombinant polyketide synthesis in Streptomyces: Engineering of improved host strains
Autore:
Ziermann, R; Betlach, MC;
Indirizzi:
KOSAN Biosci, Burlingame, CA 94010 USA KOSAN Biosci Burlingame CA USA 94010 SAN Biosci, Burlingame, CA 94010 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 1, volume: 26, anno: 1999,
pagine: 106 -
SICI:
0736-6205(199901)26:1<106:RPSISE>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROPOLYSPORA-ERYTHRAEA; BIOSYNTHESIS; SYNTHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Betlach, MC KOSAN Biosci, 1450 Rollins Rd, Burlingame, CA 94010 USA KOSAN Biosci 1450 Rollins Rd Burlingame CA USA 94010 94010 USA
Citazione:
R. Ziermann e M.C. Betlach, "Recombinant polyketide synthesis in Streptomyces: Engineering of improved host strains", BIOTECHNIQU, 26(1), 1999, pp. 106

Abstract

Efficient polyketide synthesis derived from plasmid-borne heterologous Streptomyces polyketide synthase (PKS) gene clusters necessitates a suitable host strain. Well-characterized laboratory strains such as Streptomyces coelicolor or Streptomyces lividans and their frequently used derivatives carryendogenous genes for the synthesis of actinorhodin (among other PKS genes), which might interfere with the efficient production of extrachromosomallyencoded PKS proteins and the quantitative analysis of their secreted polyketide products. To circumvent this problem, a frequently used S. coelicolorderivative, designated CH999, was engineered to lack most of the actinorhodin gene cluster. However, this strain can only be transformed with methyl-free DNA. Additionally, unlike its otherwise isogenic parent CH1, CH999 exhibits low transformation efficiencies. Here, we report the construction of two S. lividans host strains, K4-114 and K4-155. With respect to the actinorhodin gene cluster, both are genotypically identical to CH999; however, both can be transformed at considerably higher frequencies and also with methylated DNA. Upon transformation with the appropriate expression vector, CH999, K4-114 and K4-155 all produce the erythromycin precursor 6-deoxyerythronolide B(6-dEB) equally well.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 03:54:20