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Titolo:
5-oxo-6,8,11,14-eicosatetraenoic acid is a potent stimulator of L-selectinshedding, surface expression of CD11b, actin polymerization, and calcium mobilization in human eosinophils
Autore:
Powell, WS; Gravel, S; Halwani, F;
Indirizzi:
McGill Univ, Meakins Christie Labs, Dept Med, Montreal, PQ H2X 2P2, CanadaMcGill Univ Montreal PQ Canada H2X 2P2 Med, Montreal, PQ H2X 2P2, Canada McGill Univ, Dept Pathol, Montreal, PQ H2X 2P2, Canada McGill Univ Montreal PQ Canada H2X 2P2 thol, Montreal, PQ H2X 2P2, Canada
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
fascicolo: 1, volume: 20, anno: 1999,
pagine: 163 - 170
SICI:
1044-1549(199901)20:1<163:5AIAPS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; PLATELET-ACTIVATING FACTOR; HUMAN NEUTROPHILS; IN-VIVO; PHOSPHATIDYLINOSITOL 3-KINASE; SIGNAL-TRANSDUCTION; CHEMOTACTIC FACTORS; ARACHIDONIC-ACID; ADHESION; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Powell, WS McGillPQniv, Meakins Christie Labs, Dept Med, 3626 St Urbain St, Montreal, McGill Univ 3626 St Urbain St Montreal PQ Canada H2X 2P2 treal,
Citazione:
W.S. Powell et al., "5-oxo-6,8,11,14-eicosatetraenoic acid is a potent stimulator of L-selectinshedding, surface expression of CD11b, actin polymerization, and calcium mobilization in human eosinophils", AM J RESP C, 20(1), 1999, pp. 163-170

Abstract

5-ore-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) is a metabolite of arachidonic acid formed by the oxidation of 5-hydroxy-6,8,11, 14-eicosatetraenoic acid by a highly specific dehydrogenase. 5-oxo-ETE is a chemoattractant for both neutrophils and eosinophils. Although it is not as effective as leukotriene B-4 (LTB4) and platelet-activating factor (PAF) in stimulating neutrophil migration, we found that it is considerably more active than these and a variety of other Lipid mediators as an eosinophil chemoattractant. Moreover, low concentrations of 5-oxo-ETE appear to enhance the responsiveness of these cells to PAF. The objectives of the current investigation were to identify rapid responses induced in eosinophils by 5-oxo-ETE that might be related to the infiltration of these cells into tissues. We found that 5-oxo-ETE is more effective than PAF and LTB4 in inducing both L-selectin shedding and actin polymerization in hu man eosinophils, whereas PAF is the most active of these mediators in stimulating calcium mobilization. The complementary effects of 5-oxo-ETE and PAF on actin polymerization and calcium mobilization may explain their synergistic effect on eosinophil migration. 5-oxo-ETE and PAF were equipotent in stimulating the surface expression of the beta(2)-integrin CD11b, but were slightly less potent than LTB4. 5-oxo-ETE-induced actin polymerization was subject to homologous but not heterologous desensitization. It was not prevented by incubation of eosinophils withinhibitors of protein kinase C (staurosporine), mitogen-activated protein kinase kinase (PD98059), or phosphatidylinositol-3-kinase (wortmannin). In conclusion, 5-oxo-ETE is a potent activator of human eosinophils and may bean important regulator of tissue infiltration of these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:41:57