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Titolo:
Effect of experimentally induced hypothyroidism on sulfate renal transportin rats
Autore:
Sagawa, K; Murer, H; Morris, ME;
Indirizzi:
SUNY Buffalo, Dept Pharmaceut, Buffalo, NY 14260 USA SUNY Buffalo BuffaloNY USA 14260 Dept Pharmaceut, Buffalo, NY 14260 USA Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 siol, CH-8057 Zurich, Switzerland
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 1, volume: 45, anno: 1999,
pagine: F164 - F171
SICI:
0363-6127(199901)45:1<F164:EOEIHO>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUSH-BORDER MEMBRANE; THYROID-HORMONE; BASOLATERAL MEMBRANE; VESICLE PREPARATIONS; PROXIMAL TUBULE; MESSENGER-RNA; KIDNEY CORTEX; FLUIDITY; COTRANSPORT; PROTEIN;
Keywords:
inorganic sulfate; sodium sulfate cotransport; NaSi-1; kidney;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Morris, ME SUNY Buffalo, Dept Pharmaceut, 527 Hochstetter Hall, Buffalo, NY 14260 USA SUNY Buffalo 527 Hochstetter Hall Buffalo NY USA 14260 4260 USA
Citazione:
K. Sagawa et al., "Effect of experimentally induced hypothyroidism on sulfate renal transportin rats", AM J P-REN, 45(1), 1999, pp. F164-F171

Abstract

Decreased serum sulfate concentrations are observed in hypothyroid patients. However, the mechanism involved in thyroid hormone-induced alterations of renal sulfate homeostasis is unknown. The objectives of this investigation were to determine the effect of 6-propyl-2-thiouracil (PTU)-induced hypothyroidism in rats on 1) the in vivo serum concentrations, renal clearance, and renal reabsorption of sulfate, 2) the in vitro renal transport in brush-border membrane (BBM) and basolateral membrane (BLM) vesicles, and 3) the cellular mechanism of the hypothyroid-induced alteration in sulfate renal transport. Serum sulfate concentrations, renal fractional reabsorption of sulfate, and creatinine clearance were decreased significantly in the hypothyroid group. The V-max values for sodium-sulfate cotransport in BBM were significantly decreased in the kidney cortex from the hypothyroid animals (0.90 +/- 0.31 vs. 0.49 +/- 0.08 nmol.mg(-1).10 s(-1), n = 5-6, P < 0.05) without changes in K-m. There were no significant differences in V-max and K-m for sulfate/anion exchange transport in BLM. Sodium-dependent sulfate transporter (NaSi-1) mRNA and protein levels were significantly lower in the kidney cortex from hypothyroid rats. Hypothyroidism did not alter the membrane motional order (fluidity) in BBM and BLM, which indicates that the changes in the membrane fluidity do not represent the mechanism for the altered renal transport. These results demonstrate that PTU-induced hypothyroidism decreases sodium-sulfate cotransport by downregulation of the NaSi-1 gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 19:37:46