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Titolo:
Aberrant cadherin expression in bladder carcinoma cells
Autore:
Gee, J; Greathead, P; Little, AF; Libertino, JA; Summerhayes, IC;
Indirizzi:
Laheynst,chcock Med Ctr, Cell & Mol Biol Lab, Robert E Wise MD Res & Educ I Lahey Hitchcock Med Ctr Burlington MA USA 01805 rt E Wise MD Res & Educ I
Titolo Testata:
MOLECULAR UROLOGY
fascicolo: 2, volume: 2, anno: 1998,
pagine: 73 - 81
SICI:
1091-5362(199822)2:2<73:ACEIBC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULE E-CADHERIN; N-CADHERIN; ADHESION MOLECULE; BETA-CATENIN; CYTOPLASMIC DOMAIN; COMPLEMENTARY-DNA; PROSTATE-CANCER; ALPHA-CATENIN; UVOMORULIN; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Summerhayes, IC Laheynst,chcock Med Ctr, Cell & Mol Biol Lab, Robert E Wise MD Res & Educ I Lahey Hitchcock Med Ctr 31 Mall Rd Burlington MA USA 01805
Citazione:
J. Gee et al., "Aberrant cadherin expression in bladder carcinoma cells", MOL UROL, 2(2), 1998, pp. 73-81

Abstract

Altered E-cadherin expression is frequently observed in late-stage bladdercancer and is associated with a poor prognosis. Although loss of E-cadherin has been identified in 5 of 15 bladder carcinoma cell lines, the continued membrane localization of catenins in these cells suggested the expressionof alternative cadherin members. Immunoprecipitation of beta-catenin from radiolabeled cell lysates revealed coprecipitation of a 130-kD protein in cell lines lacking E-cadherin, In Western blot analysis, this putative cadherin member was found not to be P-cadherin but was immunologically related to cadherins, as revealed by reprobing of the blots with a pan-cadherin antibody. Using RT-PCR with degenerate primers targeted to a highly conserved C-terminal region of classical cadherins, we identified six cadherin membersin E-cadherin-negative cells, These proteins were P- and N-cadherin, cadherin-4, cadherin-6, cadherin-8, and a novel cadherin, designated BT-1, whichshowed significant homology with the mouse T1-cadherin, Because N-cadherinwas the most abundant sequence isolated, we confirmed expression of this protein in Western blots of cell lines and tumor lysates, Bladder cell lineslacking E- or P-cadherin showed catenin-complexed N-cadherin in all cases,Membrane localization of N-cadherin was demonstrated by immunofluorescenceof cells and bladder tumor tissue. Because N-cadherin was identified in invasive bladder carcinoma cells, it is unlikely that this cadherin member plays an invasion-suppressor role.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:46:40