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Titolo:
Development of congenics for hypnotic sensitivity to ethanol by QTL-marker-assisted counter selection
Autore:
Bennett, B; Johnson, TE;
Indirizzi:
Univ Colorado, Inst Behav Genet, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309 nst Behav Genet, Boulder, CO 80309 USA Denver Vet Adm Alcohol Res Ctr, Denver, CO 80220 USA Denver Vet Adm Alcohol Res Ctr Denver CO USA 80220 , Denver, CO 80220 USA
Titolo Testata:
MAMMALIAN GENOME
fascicolo: 12, volume: 9, anno: 1998,
pagine: 969 - 974
SICI:
0938-8990(199812)9:12<969:DOCFHS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
QUANTITATIVE TRAIT LOCI; SHORT-SLEEP MICE; RECOMBINANT INBRED STRAINS; LONG-SLEEP; MOUSE; INTERVAL; LINKAGE; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, B Univ Colorado, Inst Behav Genet, CB447, Boulder, CO 80309 USA Univ Colorado CB447 Boulder CO USA 80309 Boulder, CO 80309 USA
Citazione:
B. Bennett e T.E. Johnson, "Development of congenics for hypnotic sensitivity to ethanol by QTL-marker-assisted counter selection", MAMM GENOME, 9(12), 1998, pp. 969-974

Abstract

Mouse strains congenic for individual quantitative trait loci (QTLs) conferring hypnotic sensitivity to ethanol were constructed by backcrossing genotypically selected ILS x ISS N-2 individuals to either inbred Long Sleep (ILS) or inbred Short Sleep (ISS) mice. We used a novel "speed congenic'' approach in which N-2 mice were genotyped for markers flanking each of the five originally identified QTLs. Genotypic selection for ISS regions at four of the five QTLs, and for ILS/ISS at the fifth QTL, allowed rapid fixation of the genetic background. We call this strategy "QTL-Marker-Assisted Counter Selection" or QMACS. By the N-4 generation, phenotypic assessments showedthat in some sublines the QTL had not been captured; these sublines were discarded and positive lines split to create new replicate sublines. One QTL, on Chromosome (Chr) 8, was not confirmed. At the N-8, virtually all sublines on the remaining QTLs retained the phenotypic difference between heterozygotes and ISS homozygotes. Small numbers of interim congenics were produced at the N-6 and later generations in which the ILS QTL was made homozygous on the ISS background; as expected, these congenic mice showed an increased sleep time. For later backcrosses (after the N-6), the parents were selected on the basis of phenotype as well as genotype. The parent-offspring correlation over all QTLs was significant, supporting the use of phenotypic selection in congenic construction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:01:29