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Titolo:
Liver metastases are enhanced in homozygous deletionally mutant ICAM-1 or LFA-1 mice
Autore:
Marvin, MR; Southall, JC; Trokhan, S; DeRosa, C; Chabot, J;
Indirizzi:
Columbia Univ Coll Phys & Surg, Dept Surg, New York, NY 10032 USA ColumbiaUniv Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA
Titolo Testata:
JOURNAL OF SURGICAL RESEARCH
fascicolo: 2, volume: 80, anno: 1998,
pagine: 143 - 148
SICI:
0022-4804(199812)80:2<143:LMAEIH>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-ADHESION MOLECULE-1; MELANOMA-ASSOCIATED ANTIGEN; MONOCLONAL-ANTIBODIES; CANCER METASTASIS; TUMOR-METASTASIS; EXPRESSION; CELLS; ANGIOGENESIS; MODULATION; REJECTION;
Keywords:
metastases; ICAM-1; LFA-1; liver; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Marvin, MR Columbia32niv Coll Phys & Surg, Dept Surg, 630 W 168th St, New York, NY 100 Columbia Univ Coll Phys & Surg 630 W 168th St New York NY USA 10032
Citazione:
M.R. Marvin et al., "Liver metastases are enhanced in homozygous deletionally mutant ICAM-1 or LFA-1 mice", J SURG RES, 80(2), 1998, pp. 143-148

Abstract

Background. Adhesion molecules play an integral role in tumor growth, invasion, and metastasis and have been shown to influence the immune response to malignant cells, The interaction of intercellular adhesion molecule-1 (ICAM-1) with lymphocyte function antigen-1 (LFA-1) is important for the adhesion of leukocytes, monocytes and lymphocytes to endothelial cells in vitro and in vivo. In order to explore the role of the ICAM-1/LFA-1 interaction in liver metastases, we utilized homozygous deletionally mutant (gene knockout) mice for ICAM-1 or LFA-1 which had been derived from the C57BL6/J background. Materials and methods. Wild-type C57BL6/J mice were used as controls. Animals were anesthetized and underwent a 1-cm midline lower abdominal incision. The ileocolic vein was identified and B16 melanoma cells (10(4)) were injected. The incisions were closed with skin clips. Two weeks following surgery, mice were sacrificed and their livers resected for gross and histological analysis. Results. LFA-1 deficient mice developed 13 times the number of metastases compared to wild-type controls and ICAM-1 deficient mice developed 7 times that number [13.5 (n = 17) vs 1.0 (n = 19) and 36 (n = 10) vs 5.0 (n = 16),P values of 0.0003 and 0.0002 by Wilcoxon Rank Sum Test, respectively]. Histologically, multiple areas of inflammatory cells consisting of T-cells and macrophages were noted in wild-type mice. Only sparse inflammatory cells were noted surrounding the metastases in the null mice. Conclusions. Liver metastases of the B16 melanoma are markedly enhanced inICAM-1 null and LFA-1 null mice. The ICAM-1/LFA-1 interaction is crucial to the immune response to liver metastases. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:41:03