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Titolo:
Effects of melatonin on proliferation of cancer cell lines
Autore:
Papazisis, KT; Kouretas, D; Geromichalos, GD; Sivridis, E; Tsekreli, OK; Dimitriadis, KA; Kortsaris, AH;
Indirizzi:
Theageneecec Hosp, Virol & Tissue Culture Lab, Dept Res, Salonika 54007, Gr Theagen Canc Hosp Salonika Greece 54007 ab, Dept Res, Salonika 54007, Gr Univ Thessaly, Sch Agr, Pedion Areos, Volos, Greece Univ Thessaly Pedion Areos Volos Greece Agr, Pedion Areos, Volos, Greece Democritus Univ Thrace, Sch Med, Alexandroupolis, Greece Democritus Univ Thrace Alexandroupolis Greece , Alexandroupolis, Greece
Titolo Testata:
JOURNAL OF PINEAL RESEARCH
fascicolo: 4, volume: 25, anno: 1998,
pagine: 211 - 218
SICI:
0742-3098(199812)25:4<211:EOMOPO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PINEAL HORMONE MELATONIN; BREAST-CANCER; MICHAELIS-MENTEN; KINETIC SYSTEMS; MCF-7; GROWTH; CULTURE; INHIBITIONS; CARCINOMA; FORMAZAN;
Keywords:
melatonin; cancer; in vitro; sulforhodamine; SRB; cell lines bromodeoxyuridine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Papazisis, KT Theagen,Canc Hosp, Virol & Tissue Culture Lab, Dept Res, Al Simeonidi Str 2 Theagen Canc Hosp Al Simeonidi Str 2 Salonika Greece 54007
Citazione:
K.T. Papazisis et al., "Effects of melatonin on proliferation of cancer cell lines", J PINEAL R, 25(4), 1998, pp. 211-218

Abstract

The pineal hormone melatonin has been reported to have in vitro antiproliferative effects on estrogen receptor-positive human breast cancer cell lines at concentrations near to plasma physiological concentrations (1 x 10(-11) to 1 x 10(-9) M). Its growth inhibitory actions have been thought to be linked to the estrogen-receptor system. We tested the cytotoxic effects of melatonin on MCF-7 and T47D human breast cancer cell lines by using the SRB (sulforhodamine-B), XTT-tetrazolium, and bromodeoxyuridine (BrdU) assays in96-well microtiter plates. After a 3 or 4 day exposure, melatonin did not have any significant effect on breast cancer cell proliferation and survival in doses up to 1 x 10(-4) M. Doses higher than 1 mM exhibited a potent cytotoxic effect, which was not mediated by the estrogen-receptor or by protein tyrosine kinases and was not specific for breast cancer cell lines. Intracellular glutathione levels did not seem to play any role: in the sensitivity of breast cancer cells to melatonin, since the addition of L-buthionine-[S,R]-sulfoximine, ethacrynic acid, or exogenous glutathione did not modify our results. We conclude that under our experimental conditions melatoninhas no inhibitory effects on human breast cancer cells at low (physiological or supraphysiological) concentrations. The different experimental procedures that were utilized in the pre:;ent study can partially explain the divergence between our results and the literature.

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Documento generato il 05/07/20 alle ore 09:10:52