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Titolo:
Dithiocarbamate pesticides affect glutamate transport in brain synaptic vesicles
Autore:
Vaccari, A; Saba, P; Mocci, I; Ruiu, S;
Indirizzi:
Univ,Cagliari, Bb Brodie Dept Neurosci, Neurotoxicol Unit, I-09124 Cagliari Univ Cagliari Cagliari Italy I-09124 Neurotoxicol Unit, I-09124 Cagliari
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 1, volume: 288, anno: 1999,
pagine: 1 - 5
SICI:
0022-3565(199901)288:1<1:DPAGTI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYLENE-BIS-DITHIOCARBAMATE; EXTRACELLULAR GLUTAMATE; NEUROLOGICAL DISORDERS; CHRONIC EXPOSURE; FUNGICIDE MANEB; NEURONAL DAMAGE; RAT-BRAIN; NEUROTOXICITY; ACCUMULATION; DIETHYLDITHIOCARBAMATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Vaccari, A Univ-09124ari, Bb Brodie Dept Neurosci, Neurotoxicol Unit, Via Porcell 4, I Univ Cagliari Via Porcell 4 Cagliari Italy I-09124 orcell 4, I
Citazione:
A. Vaccari et al., "Dithiocarbamate pesticides affect glutamate transport in brain synaptic vesicles", J PHARM EXP, 288(1), 1999, pp. 1-5

Abstract

Dithiocarbamate compounds are widely used agricultural fungicides that display low acute toxicity in mammals and that may become neurotoxic after prolonged exposure. Mancozeb, among other dithiocarbamates tested, proved to be the most potent (K-i = 0.27 mu M) at noncompetitively inhibiting the in vitro ATP-dependent uptake of [H-3]glutamate in rat cortical vesicles. Furthermore, mancozeb partially (20%) inhibited the ATP-dependent uptake of [C-14]methylamine, used as an index for the vesicular transmembrane proton gradient (Delta pH), and evoked its efflux from organelles previously incubatedwith the H-3-labeted marker. Meanwhile, the vesicular uptake of (36)chloride(-) anions whose concentrations regulate the transmembrane potential gradient (Delta psi(SV)) was not impaired. The dithiocarbamate effects on the vesicular transport of [H-3]glutamate thus appeared to involve mainly the Delta pH gradient rather than the potential gradient. Dithiocarbamate metabolites, the potent neurotoxin carbon disulfide included, did not affect the uptake process, thus implying the relevance for inhibition of the persistence, if any, of parent compounds in the brain. The present novel and potent in vitro interferences of selected dithiocarbamate pesticides with the vesicular transport of glutamate, if representative of in vivo alterations, may play some role in the probably complex origin of dithiocarbamate neurotoxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:18:10