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Titolo:
Dual mode of interaction of DNA polymerase epsilon with proliferating cellnuclear antigen in primer binding and DNA synthesis
Autore:
Maga, G; Jonsson, ZO; Stucki, M; Spadari, S; Hubscher, U;
Indirizzi:
CNR, Inst Biochem & Evolut Genet, IGBE, I-27100 Pavia, Italy CNR Pavia Italy I-27100 ochem & Evolut Genet, IGBE, I-27100 Pavia, Italy Univ Zurich Irchel, Inst Vet Biochem, CH-8057 Zurich, Switzerland Univ Zurich Irchel Zurich Switzerland CH-8057 H-8057 Zurich, Switzerland
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 1, volume: 285, anno: 1999,
pagine: 259 - 267
SICI:
0022-2836(19990108)285:1<259:DMOIOD>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION-FACTOR-C; CALF THYMUS; AUXILIARY PROTEINS; ESCHERICHIA-COLI; DELTA; REPAIR; ALPHA; PCNA; RECONSTITUTION; SUBUNIT;
Keywords:
DNA replication; DNA polymerase; processivity factor; protein-protein interaction; PCNA mutagenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Maga, G CNR,a,nst Biochem & Evolut Genet, IGBE, Via Abbiategrasso 207, I-27100 Pavi CNR Via Abbiategrasso 207 Pavia Italy I-27100 o 207, I-27100 Pavi
Citazione:
G. Maga et al., "Dual mode of interaction of DNA polymerase epsilon with proliferating cellnuclear antigen in primer binding and DNA synthesis", J MOL BIOL, 285(1), 1999, pp. 259-267

Abstract

Proliferating cell nuclear antigen can interact with DNA polymerase epsilon on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication protein A), and thereby stimulate its primer recognition and DNA synthesis. Using four characterized mutants of proliferating cell nuclear antigen containing three or four alanine residue substitutions on the C-terminal side and the back side of the trimer, we have tested the kinetics of primer binding and nucleotide incorporation by DNA polymerase epsilon in different assays. In contrast with what has been foundin interaction studies between DNA polymerase delta and proliferating cellnuclear antigen, our data suggested that stimulation of DNA polymerase epsilon primer binding involves interactions with both the C-terminal side andthe back side of proliferating cell nuclear antigen. However, for stimulation of DNA polymerase epsilon DNA synthesis, exclusively the C-terminal side appears to be sufficient. The significance of this dual interaction is discussed with reference to the physiological roles of DNA polymerase epsilonand its interaction with the clamp proliferating cell nuclear antigen. (C)1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:01:29