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Titolo:
Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells
Autore:
Gorczynski, L; Chen, Z; Hu, J; Kai, Y; Lei, J; Ramakrishna, V; Gorczynski, RM;
Indirizzi:
Toronto Hosp, Transplant Res Div, CCRW 2 855, Toronto, ON M5G 2C4, Canada Toronto Hosp Toronto ON Canada M5G 2C4 2 855, Toronto, ON M5G 2C4, Canada
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 162, anno: 1999,
pagine: 774 - 781
SICI:
0022-1767(19990115)162:2<774:ETAOCC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORTAL VENOUS IMMUNIZATION; ANTIGEN-PRESENTING CELLS; T-CELLS; ALLOGRAFT-REJECTION; GRAFT PROLONGATION; PERIPHERAL-BLOOD; ALLOGENEIC CELLS; SKIN ALLOGRAFTS; NITRIC-OXIDE; IFN-GAMMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Gorczynski, RM Toronto Hosp, Transplant Res Div, CCRW 2 855, 200 ElizabethSt, Toronto, ON Toronto Hosp 200 Elizabeth St Toronto ON Canada M5G 2C4 ON
Citazione:
L. Gorczynski et al., "Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells", J IMMUNOL, 162(2), 1999, pp. 774-781

Abstract

We reported that hepatic mononuclear, nonparenchymal cells (NPC) can inhibit the immune response seen when allogeneic C57BL/6 dendritic cells (DC) are incubated with C3H spleen responder cells. Cells derived from these cultures transfer increased survival of C57BL/6 renal allografts in C3H mice. Wealso found that increased expression of OX-2 on DC was associated with inhibition of cytokine production and renal allograft rejection. We explored whether inhibition by hepatic NPC was a function of OX-2 expression by thesecells. Fresh C57BL/6 spleen-derived DC were cultured with C3H spleen responder cells and other putative coregulatory cells. The latter were derived from fresh C3H or C57BL/6 liver NPC, or from C3H or C57BL/6 mice treated for10 days by i.v. infusion of human Flt3 ligand, Different populations of murine bone marrow-derived DC from cultures of bone marrow with IL-4 plus granulocyte macrophage-CSF were also used as a source of putative regulator cells. Supernatants of all stimulated cultures were examined for functional expression of different cytokines (IL-2, IL-4, IFN-gamma, and TGF beta). We found that fresh C57BL/6 splenic DC induced IL-2, not IL-4, production, Cells from the sources indicated inhibited IL-2 and IFN-gamma production and promoted IL-4 and TGF beta production. Inhibition was associated with increased expression of OX-2 on these cells, as defined by semiquantitative PCR and FAGS analysis. By size fractionation, cells expressing OX-2 were a subpopulation of NLDC145(+) cells. Our data imply a role for cells expressing OX-2 in the regulation of induction of cytokine production by conventional allostimulatory DC.

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Documento generato il 16/07/20 alle ore 05:56:00