Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A standardized, computer-assisted in vitro assay for the assessment of neutrophil transmigration across endothelial monolayers
Autore:
Groger, M; Matsumura, T; Kohrgruber, N; Maurer, D; Wolff, K; Petzelbauer, P;
Indirizzi:
Univ Vienna, Sch Med, Div Gen Dermatol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 v Gen Dermatol, A-1090 Vienna, Austria Univriaenna, Sch Med, Div Immunol Allergy & Infect Dis, A-1090 Vienna, Aust Univ Vienna Vienna Austria A-1090 ergy & Infect Dis, A-1090 Vienna, Aust
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 222, anno: 1999,
pagine: 101 - 109
SICI:
0022-1759(19990101)222:1-2<101:ASCIVA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE ADHESION; CELL JUNCTIONS; E-SELECTIN; MIGRATION; RECEPTORS; PECAM-1; ANTIGEN; ELAM-1; ACTIN;
Keywords:
transmigration assay; endothelium; neutrophils;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Petzelbauer, P Univenna,na, Sch Med, Div Gen Dermatol, Waehringer Guertel 18-20, A-1090 Vi Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090
Citazione:
M. Groger et al., "A standardized, computer-assisted in vitro assay for the assessment of neutrophil transmigration across endothelial monolayers", J IMMUNOL M, 222(1-2), 1999, pp. 101-109

Abstract

We describe an automated, observer-independent and highly reproducible assay for the quantification of transmigrated neutrophils across endothelial monolayers. Endothelial cells grown on collagen gels were loaded with a dye emitting red fluorescence. Neutrophils loaded with dye emitting green fluorescence were allowed to adhere to and transmigrate across endothelial monolayers. For quantification of adherent and migrated cells? randomly selectedfields were scanned by confocal laser scan microscopy at defined depths within and below the endothelial monolayers. The images obtained were transferred into the public domain NIH image program and numbers and distribution of cells within scanned sectors were automatically calculated. We demonstrate that adherent neutrophils are easily discriminated from transmigrated cells; absolute numbers of migrated cells can be reproducibly calculated by counting cells at a depth of - 20 mu m: thus permitting evaluation of large-scale experiments; the efficacy of neutrophil transmigration depends on thelevel of endothelial activation after TNF stimulation and mAbs to cell surface adhesion molecules interfere with migration in a manner similar to that previously shown in in vivo experiments, This assay lends itself to the identification of molecules influencing in cell migration in each phase of EC activation and to the screening of pro- and anti-migratory properties of biological or pharmacological reagents. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:11:49