Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Proliferation of endothelial cells on surface-immobilized albumin-heparin conjugate loaded with basic fibroblast growth factor
Autore:
Bos, GW; Scharenborg, NM; Poot, AA; Engbers, GHM; Beugeling, T; van Aken, WG; Feijen, T;
Indirizzi:
Univede,nte, Fac Chem Technol, Sect Polymer Chem & Biomat, NL-7500 AE Ensch Univ Twente Enschede Netherlands NL-7500 AE m & Biomat, NL-7500 AE Ensch Univ Twente, Inst Biomed Technol, NL-7500 AE Enschede, Netherlands Univ Twente Enschede Netherlands NL-7500 AE 500 AE Enschede, Netherlands
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 3, volume: 44, anno: 1999,
pagine: 330 - 340
SICI:
0021-9304(19990305)44:3<330:POECOS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEOLYTIC DEGRADATION; EXTRACELLULAR-MATRIX; VASCULAR GRAFTS; BINDING; DACRON; DIFFERENTIATION; ANGIOGENESIS; PROSTHESIS; PROTEINS; RELEASE;
Keywords:
small-diameter vascular grafts; endothelial cell seeding; albumin-heparin conjugate; basic fibroblast growth factor (bFGF); stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Feijen, T Univede,nte, Fac Chem Technol, Sect Polymer Chem & Biomat, NL-7500 AE Ensch Univ Twente Enschede Netherlands NL-7500 AE , NL-7500 AE Ensch
Citazione:
G.W. Bos et al., "Proliferation of endothelial cells on surface-immobilized albumin-heparin conjugate loaded with basic fibroblast growth factor", J BIOMED MR, 44(3), 1999, pp. 330-340

Abstract

Seeding of endothelial cells (ECs) on the luminal surface of small-diameter vascular grafts is a promising method to avoid occlusion of these prostheses. Immobilization of basic fibroblast growth factor (bFGF) to substrates used to coat or fill porous prostheses may enhance the formation of a confluent monolayer of ECs. Human umbilical vein endothelial cells (HUVECs) weregrown on bFGF-loaded albumin-heparin conjugate bound to CO2 gas-plasma-treated polystyrene. In the order of 2-3 ng/cm(2) bFGF had to be immobilized to form a confluent monolayer of HUVECs. The most prominent effect of surface-immobilized bFGF was stimulation of the proliferation shortly after seeding, resulting within 3 days in confluent cell monolayers with high density. In contrast, in cultures with 0.3 ng/mL bFGF in the medium instead of bFGFbound to the surface, it took almost a week before the cell layers reachedconfluency. Binding of bFGF to heparin and the biological activity of bFGFtowards ECs were not influenced by the (radio-)labeling of bFGF with iodine. However, only a minor part of the bFGF used in this study displayed heparin affinity. Furthermore, degradation and multimerization of labeled bFGF in time occurred when the growth factor was stored at 20 degrees-37 degreesC. This Limits the use of labeled bFGF to short-term (hours) experiments. In conclusion, bFGF loading of vascular graft surfaces through complexationof bFGF with a heparin-containing matrix probably will lead to more rapid formation of a confluent monolayer of ECs on graft surfaces upon seeding ofthe cells. (C) 1999 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:37:51