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Titolo:
PJA-BP expression and TCR delta deletion during human T cell differentiation
Autore:
Verschuren, MCM; Blom, B; Bogers, AJJC; Spits, H; van Dongen, JJM;
Indirizzi:
Erasmus Univ, Dept Immunol, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands Univ Rotterdam Hosp, NL-3000 DR Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3000 DR terdam, Netherlands Netherlands Canc Inst, Div Immunol, NL-1066 CX Amsterdam, Netherlands Netherlands Canc Inst Amsterdam Netherlands NL-1066 CX rdam, Netherlands Erasmus Univ, Dept Thorac Surg, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 12, volume: 10, anno: 1998,
pagine: 1873 - 1880
SICI:
0953-8178(199812)10:12<1873:PEATDD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN THYMIC DEVELOPMENT; LYMPHOCYTES-T; RECEPTOR; ALPHA; THYMOCYTES; GENE; REARRANGEMENTS; LOCUS; CD3; DNA;
Keywords:
human thymocytes; (Psi)J(alpha) binding protein; PJA-BP; recombination; T cell differentiation; TCR delta gene deletion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: van Dongen, JJM Erasmus Univ, Dept Immunol, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam Netherlands NL-3000 DR ds
Citazione:
M.C.M. Verschuren et al., "PJA-BP expression and TCR delta deletion during human T cell differentiation", INT IMMUNOL, 10(12), 1998, pp. 1873-1880

Abstract

Recombination of delta Rec to psi J(alpha) will delete the TCR delta gene,which is thought to play an important role in the bifurcation of the TCR alpha beta versus TCR gamma delta differentiation lineages. We recently detected a DNA-binding protein in human thymocytes, the so-called PJA-BP, whichrecognizes the psi J(alpha) gene segment and might be one of the factors involved in the regulation of preferential delta Rec-psi J(alpha) rearrangements. We now investigate PJA-BP expression and its correlation with TCR delta gene deletion in thymocytes. Our electrophoretic mobility shift assay experiments showed that the PJA-BP is evolutionary conserved in human, murineand simian thymocytes. Using a large series of human hematopoietic malignancies (n = 30), we conclude that PJA-BP expression is thymocyte specific and seems to be restricted to thymocytes committed to the TCR alpha beta lineage, Analysis of seven well-defined human thymocyte subpopulations showed that preferential delta Rec-psi J(alpha) rearrangements as well as PJA-BP expression can be detected from the immature CD34(-)/CD1(+)/CD3(-)/CD4(+)/CD8alpha(+)beta(-) thymocyte differentiation stage onwards. These experimentsindicate that expression of PJA-BP in human thymocytes starts simultaneously with preferential delta Rec-psi J(alpha) rearrangements, which supports our hypothesis that PJA-BP is one of the factors involved in the preferential recombination of delta Rec to psi J(alpha).

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Documento generato il 05/04/20 alle ore 20:33:45