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Titolo:
A LINEAR B-CELL EPITOPE AN THE CLASS-3 OUTER-MEMBRANE PROTEIN OF NEISSERIA-MENINGITIDIS RECOGNIZED AFTER VACCINATION WITH THE NORWEGIAN GROUP-B OUTER-MEMBRANE VESICLE VACCINE
Autore:
DELVIG AA; WEDEGE E; CAUGANT DA; DALSEG R; KOLBERG J; ACHTMAN M; ROSENQVIST E;
Indirizzi:
NATL INST PUBL HLTH,DEPT VACCINES N-0462 OSLO NORWAY NATL INST PUBL HLTH,DEPT BACTERIOL N-0462 OSLO NORWAY MAX PLANCK INST MOLEC GENET D-14195 BERLIN GERMANY
Titolo Testata:
Microbiology
, volume: 141, anno: 1995,
parte:, 7
pagine: 1593 - 1600
SICI:
1350-0872(1995)141:<1593:ALBEAT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; MONOCLONAL-ANTIBODIES; BACTERICIDAL ANTIBODIES; MENINGOCOCCAL DISEASE; SYNTHETIC PEPTIDES; STRAINS; IDENTIFICATION; SEROTYPES; SEQUENCES; ANTIGENS;
Keywords:
NEISSERIA MENINGITIDIS; PORB PROTEIN; B-CELL EPITOPE; MURINE MABS; SYNTHETIC PEPTIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
A.A. Delvig et al., "A LINEAR B-CELL EPITOPE AN THE CLASS-3 OUTER-MEMBRANE PROTEIN OF NEISSERIA-MENINGITIDIS RECOGNIZED AFTER VACCINATION WITH THE NORWEGIAN GROUP-B OUTER-MEMBRANE VESICLE VACCINE", Microbiology, 141, 1995, pp. 1593-1600

Abstract

The class 3 outer-membrane protein (OMP) of Neisseria meningitidis isa potential target for bactericidal and opsonic antibodies in humans. Synthetic peptides spanning the class 3 OMP from the vaccine strain 44/76 (B:15:P1.7.16:L3,7) were synthesized on pins and screened with serum obtained from Norwegian adolescents immunized with a meningococcalserogroup B outer-membrane vesicle (OMV) vaccine. A strong IgG response to a single peptide ((19)FHQNGQVTEVTT(30)) located within loop 1 (VR1) was stimulated after three doses of OMV vaccine in three vaccineesselected on the basis of their antibody response to class 3 OMP. No clear linear B-cell epitopes were recognized by four different murine serotype 15-specific mAbs. A 23mer peptide (D63b2) containing loop 1 ofthe class 3 OMP was synthesized, and the IgG responses were measured in pre- and post-vaccination serum from 27 vaccinees. Specific IgG rose significantly in 37% of vaccinees 6 weeks after the second dose and in 74% of the vaccinees 6 weeks after the third dose of the OMV vaccine. Most immune sera reacted distinctly on immunoblots with denatured class 3 OMP, and the immunoblotting reactivity correlated strongly withconcentration of the IgG antibodies specific for peptide D63b2. When added to a post-vaccination serum from one vaccinee, peptide D63b2 competed efficiently with the class 3 OMP for specific antibody binding on immunoblots and in pin ELISA. The results show that the significant part of the humoral response to the meningococcal class 3 OMP elicitedby vaccination with the Norwegian OMV vaccine was directed against a single continuous epitope.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:31:31