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Titolo:
In vitro characterization of the tobacco rpoB promoter reveals a core sequence motif conserved between phage-type plastid and plant mitochondrial promoters
Autore:
Liere, K; Maliga, P;
Indirizzi:
Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Inst, Piscataway, NJ 08854 USA
Titolo Testata:
EMBO JOURNAL
fascicolo: 1, volume: 18, anno: 1999,
pagine: 249 - 257
SICI:
0261-4189(19990104)18:1<249:IVCOTT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLOROPLAST RNA-POLYMERASE; TRANSCRIPTION INITIATION SITES; RIBOSOME-DEFICIENT PLASTIDS; SINAPIS-ALBA L; ACCURATE TRANSCRIPTION; ARABIDOPSIS-THALIANA; NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; GENE-EXPRESSION; INVITRO SYSTEM;
Keywords:
in vitro transcription; nuclear-encoded plastid RNA polymerase; phage-type RNA polymerases; plastid promoters; rpoB operon;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Maliga, P Rutgers4State Univ, Waksman Inst, 190 Frelinghuysen Rd, Piscataway, NJ 0885 Rutgers State Univ 190 Frelinghuysen Rd Piscataway NJ USA 08854
Citazione:
K. Liere e P. Maliga, "In vitro characterization of the tobacco rpoB promoter reveals a core sequence motif conserved between phage-type plastid and plant mitochondrial promoters", EMBO J, 18(1), 1999, pp. 249-257

Abstract

We report here the in vitro characterization of PrpoB-345, the tobacco rpoB promoter recognized by NEP, the phage-type plastid RNA polymerase. Transcription extracts were prepared from mutant tobacco plants lacking PEP, the Escherichia coli-like plastid-encoded RNA polymerase. Systematic dissectionof a similar to 1 kb fragment determined that the rpoB promoter is contained in a 15-nucleotide segment (-14 to + 1) upstream of the transcription initiation site ( + 1), Point mutations at every nucleotide reduced transcription, except at the -5 position which was neutral. Critical for rpoB promoter function was a CRT-motif (CAT or CGT) at -8 to -6 (transcription <30%), defining it as the promoter core. The core CAT sequence is also present in the maize rpoB promoter, which is faithfully recognized by tobacco extracts. Alignment of NEP promoters identified a CATA or TATA (= YATA) sequence atthe rpoB core position, also present in plant mitochondrial promoters. Furthermore, NEP and the phage T7 RNA polymerase exhibit similar sensitivity to inhibitors of transcription. These data indicate that the nuclear RpoZ gene, identified by sequence conservation with mitochondrial RNA polymerases,encodes the NEP catalytic subunit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 14:15:00