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Titolo:
Decreased mitochondrial DNA content in peripheral blood precedes the development of non-insulin-dependent diabetes mellitus
Autore:
Lee, HK; Song, JH; Shin, CS; Park, DJ; Park, KS; Lee, KU; Koh, CS;
Indirizzi:
Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 Med, Seoul 110744, South Korea Natl Inst Hlth, Dept Biomed Sci, Eunpyung Ku, Seoul 122020, South Korea Natl Inst Hlth Seoul South Korea 122020 ng Ku, Seoul 122020, South Korea Univ Ulsan, Coll Med, Dept Internal Med, Seoul, South Korea Univ Ulsan Seoul South Korea Med, Dept Internal Med, Seoul, South Korea
Titolo Testata:
DIABETES RESEARCH AND CLINICAL PRACTICE
fascicolo: 3, volume: 42, anno: 1998,
pagine: 161 - 167
SICI:
0168-8227(199812)42:3<161:DMDCIP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRNA(LEU(UUR)) GENE; YONCHON COUNTY; POINT MUTATION; DEAFNESS; KOREA;
Keywords:
mitochondrial DNA; peripheral blood; non-insulin-dependent diabetes mellitus; waist-to-hip ratio; blood pressure; insulin resistance; pre-diabetes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, HK Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 oul 110744, South Korea
Citazione:
H.K. Lee et al., "Decreased mitochondrial DNA content in peripheral blood precedes the development of non-insulin-dependent diabetes mellitus", DIABET RE C, 42(3), 1998, pp. 161-167

Abstract

Qualitative changes in mitochondrial DNA (mtDNA), such as mutations and deletions, have been implicated in the pathogenesis of diabetes mellitus. In addition to the qualitative changes, mtDNA is subject to quantitative changes, and is vulnerable to oxidative stress, resulting in both qualitative and quantitative changes. This study was performed to investigate whether quantitative changes in mtDNA occur in non-insulin-dependent diabetes mellitus(NIDDM) patients and also in pre-diabetic subjects. MtDNA content from peripheral blood was measured by slot-blot analysis in 55 NIDDM patients and 29 age- and sex-matched control subjects. We have also analysed the mtDNA copies by quantitative polymerase chain reaction (PCR) method in 23 pre-diabetic subjects who converted to diabetic in 2 years and 12 age- and sex-matched control subjects who remained non-diabetic. Mean mtDNA quantity measuredby slot blot method was 35% lower in patients with NIDDM than in control subjects (12.3 +/- 8.1 vs. 19.1 +/- 8.2 AU/mu g DNA; P < 0.05). MtDNA quantities did not correlate with age, body mass index, duration of diabetes or HbA(1c) levels. We have also found that the mtDNA copies in subjects who converted to diabetes in 2 years were lower than in controls even before the development of diabetes (102.8 +/- 41.5 vs. 137.8 +/- 67.7 copies/pg template DNA P(0.05). Inverse correlations were noted between mtDNA content and baseline waist hip circumference ratio (WHR) (r = -0.31, P < 0.05), and fasting glucose level (r = -0.35, P < 0.05), diastolic blood pressure (r = -0.36, P < 0.05), and WHR (r = -0.40, P < 0.01) after development of diabetes. In conclusion, we demonstrate that the content of mtDNA decreases in peripheral blood of patients with NIDDM and the lower mtDNA levels precede the development of diabetes. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 13:28:08