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Titolo:
Effects of Shh and Noggin on neural crest formation demonstrate that BMP is required in the neural tube but not ectoderm
Autore:
Selleck, MAJ; Garcia-Castro, MI; Artinger, KB; Bronner-Fraser, M;
Indirizzi:
CALTECH 139 74, Div Biol, Pasadena, CA 91125 USA CALTECH 139 74 Pasadena CA USA 91125 74, Div Biol, Pasadena, CA 91125 USA CALTECH 139 74, Beckman Inst, Pasadena, CA 91125 USA CALTECH 139 74 Pasadena CA USA 91125 Beckman Inst, Pasadena, CA 91125 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 24, volume: 125, anno: 1998,
pagine: 4919 - 4930
SICI:
0950-1991(199812)125:24<4919:EOSANO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPING SPINAL-CORD; CELL LINEAGE ANALYSIS; FLOOR PLATE; CHICK-EMBRYO; SONIC-HEDGEHOG; EXPRESSION PATTERNS; POLARIZING ACTIVITY; NERVOUS-SYSTEM; AVIAN EMBRYO; HENSENS NODE;
Keywords:
neural crest cells; notochord; slug; neural plate; commitment; chick;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Selleck, MAJ Univoso Calif, Sch Med, Dept Cell & Neurobiol, 1333 San PabloSt,BMT 401, L Univ So Calif 1333 San Pablo St,BMT 401 Los Angeles CA USA 90033
Citazione:
M.A.J. Selleck et al., "Effects of Shh and Noggin on neural crest formation demonstrate that BMP is required in the neural tube but not ectoderm", DEVELOPMENT, 125(24), 1998, pp. 4919-4930

Abstract

To define the timing of neural crest formation, we challenged the fate of presumptive neural crest cells by grafting notochords, Sonic Hedgehog- (Shh) or Noggin-secreting cells at different stages of neurulation in chick embryos. Notochords or Shh-secreting cells are able to prevent neural crest formation at open neural plate levels, as assayed by DiI-labeling and expression of the transcription factor, Slug, suggesting that neural crest cells are not committed to their fate at this time. In contrast, the BMP signalingantagonist, Noggin, does not repress neural crest formation at the open neural plate stage, but does so if injected into the lumen of the closing neural tube. The period of Noggin sensitivity corresponds to the time when BMPs are expressed in the dorsal neural tube but are down-regulated in the non-neural ectoderm, To confirm the timing of neural crest formation, Shh or Noggin were added to neural folds at defined times in culture, Shh inhibits neural crest production at early stages (0-5 hours in culture), whereas Noggin exerts an effect on neural crest production only later (5-10 hours in culture). Our results suggest three phases of neurulation that relate to neural crest formation: (1) an initial BMP-independent phase that can be prevented by Shh-mediated signals from the notochord; (2) an intermediate BMP-dependent phase around the time of neural tube closure, when BMP-4 is expressed in the dorsal neural tube; and (3) a later premigratory phase which is refractory to exogenous Shh and Noggin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 12:44:28