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Titolo:
DISRUPTION OF RIBOSOMAL SCANNING ON THE 5'-UNTRANSLATED REGION, AND NOT RESTRICTION OF TRANSLATIONAL INITIATION PER SE, MODULATES THE STABILITY OF NONABERRANT MESSENGER-RNAS IN THE YEAST SACCHAROMYCES-CEREVISIAE
Autore:
LINZ B; KOLOTEVA N; VASILESCU S; MCCARTHY JEG;
Indirizzi:
UMIST,DEPT BIOMOL SCI,POB 88 MANCHESTER M60 1QD LANCS ENGLAND UMIST,DEPT BIOMOL SCI MANCHESTER M60 1QD LANCS ENGLAND
Titolo Testata:
The Journal of biological chemistry
fascicolo: 14, volume: 272, anno: 1997,
pagine: 9131 - 9140
SICI:
0021-9258(1997)272:14<9131:DORSOT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA DEGRADATION; MAMMALIAN-CELLS; BINDING-PROTEIN; ENDONUCLEOLYTIC CLEAVAGE; SECONDARY STRUCTURE; NONSENSE MUTATIONS; FIREFLY LUCIFERASE; CODING REGION; INHIBITION; DECAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
B. Linz et al., "DISRUPTION OF RIBOSOMAL SCANNING ON THE 5'-UNTRANSLATED REGION, AND NOT RESTRICTION OF TRANSLATIONAL INITIATION PER SE, MODULATES THE STABILITY OF NONABERRANT MESSENGER-RNAS IN THE YEAST SACCHAROMYCES-CEREVISIAE", The Journal of biological chemistry, 272(14), 1997, pp. 9131-9140

Abstract

Translation and mRNA decay constitute key players in the post-transcriptional control of gene expression. We examine the mechanisms by which the 5'-untranslated region (UTR) of nonaberrant mRNAs acts to modulate both these processes in Saccharomyces cerevisiae. Two classes of functional relationship between ribosome-5'-UTR interactions and mRNA decay are identifiable. In the first of these, elements in the main openreading frame (ORF) dictate how the decay process reacts to inhibitory structures in the 5'-UTR. The same types of stability modulation canbe elicited by transregulation of translation via inducible binding of the iron-regulatory protein to an iron-responsive element located 9 nucleotides from the 5' cap. A eukaryotic translational repressor can therefore modulate mRNA decay via the 5'-UTR. In contrast, translational regulation mediated via changes in the activity of the cap-binding eukaryotic translation initiation factor eIF-4E bypasses translation-dependent pathways of mRNA degradation. Thus modulation of mRNA stability via the 5'-UTR depends on disruption of the scanning process, rather than changes in translational initiation efficiency per se. In the second class of pathway, an upstream ORF (uORF) functions as a powerfuldestabilizing element, inducing termination-dependent degradation that is apparently independent of any main ORF determinants but influenced by the efficiencies of ribosomal recognition of the uORF start and stop codons. This latter mechanism provides a regulatable means to modulate the stability of nonaberrant mRNAs via a UPF-dependent pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 22:54:52