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Titolo:
Comparison of the phagocytosis of two types of cyclosporin (SDZ OXL 400 and SDZ IMM 125) by alveolar macrophages from hamsters
Autore:
Maye, I; de Fraissinette, A; Cruz-Orive, LM; Andersen, E; Niederlander, C; Bueb, W; Froehlich, C; Schreier, M; Vonderscher, J; Richter, F; Cordier, A; Gehr, P;
Indirizzi:
Novartis Pharma, Preclin Safety, Toxicol Pathol, CH-4002 Basel, Switzerland Novartis Pharma Basel Switzerland CH-4002 ol, CH-4002 Basel, Switzerland Univ Bern, Inst Anat, CH-3012 Bern, Switzerland Univ Bern Bern Switzerland CH-3012 Inst Anat, CH-3012 Bern, Switzerland Univ Cantabria, Dept Math, E-39005 Santander, Spain Univ Cantabria Santander Spain E-39005 pt Math, E-39005 Santander, Spain
Titolo Testata:
CELL BIOLOGY AND TOXICOLOGY
fascicolo: 6, volume: 14, anno: 1998,
pagine: 411 - 418
SICI:
0742-2091(199812)14:6<411:COTPOT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONONUCLEAR PHAGOCYTES; GROWTH-FACTOR; SEVERE ASTHMA; ACTIVATION; CLEARANCE; PARTICLES; PULMONARY; RESPONSES; THERAPY; OXIDE;
Keywords:
cell activation; cell viability; cyclosporin; phagocytosis; stereology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: de Fraissinette, A Novartisl,harma, Preclin Safety, Toxicol Pathol, Bldg 881-330, CH-4002 Base Novartis Pharma Bldg 881-330 Basel Switzerland CH-4002
Citazione:
I. Maye et al., "Comparison of the phagocytosis of two types of cyclosporin (SDZ OXL 400 and SDZ IMM 125) by alveolar macrophages from hamsters", CELL BIOL T, 14(6), 1998, pp. 411-418

Abstract

The aim of this study was to compare two types of cyclosporin (Cs) particles, SDZ OXL 400 and SDZ IMM 125, the latter being more hydrophilic, to understand their uptake by airway macrophages. Alveolar macrophages (AM), harvested by bronchoalveolar lavage (BAL) of hamster lungs, were cultured with two different doses (0.1 mg and 0.5 mg) for 1 h, 6 h, and 24 h. Control incubations without Cs particles or with latex particles were carried out simultaneously. Cell viability, cell activation (i.e., respiratory burst, interleukin-6 (IL-6) synthesis) and mean volume of particles phagocytosed per macrophage were measured. Both types of Cs particles did not modify the AM viability, and failed to induce IL-6 synthesis during phagocytosis but slightly decreased the cell oxidative respiratory burst. The comparison between SDZ OXL 400 and SDZ IMM 125 showed that for the lower dose the mean volume ofboth Cs types phagocytosed was similar at 1 h and 6 h. At 24 h an increaseof the mean volume phagocytosed was seen for SDZ IMM 125 but not for SDZ OXL 400. For the higher dose the mean volume of SDZ IMM 125 phagocytosed washigher than SDZ OXL 400 at I h and 6 h and comparable for both types at 24h. SDZ IMM 125 particles were phagocytosed more rapidly than SDZ OXL 400. The mean volume of phagocytosed latex particles increased with time and dose and was higher than for both Cs particle types. In conclusion, AM were seen to phagocytose particles of different physical properties (i.e., form, size, and shape), chemical properties (i.e., inert or peptidic) and degrees of hydrophilicity in a different manner.

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Documento generato il 29/09/20 alle ore 16:08:14