Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of postmortem delay on serotonin and (+)8-OH-DPAT-mediated inhibition of adenylyl cyclase activity in rat and human brain tissues
Autore:
Palego, L; Giromella, A; Marazziti, D; Borsini, F; Naccarato, AG; Giannaccini, G; Lucacchini, A; Cassano, GB; Mazzoni, MR;
Indirizzi:
UnivyPisa, Dept Psichiat Neurobiol Farmacol & Biotecnol, I-56100 Pisa, Ital Univ Pisa Pisa Italy I-56100 ol Farmacol & Biotecnol, I-56100 Pisa, Ital Univ Pisa, Dept Oncol, I-56100 Pisa, Italy Univ Pisa Pisa Italy I-56100Univ Pisa, Dept Oncol, I-56100 Pisa, Italy
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1, volume: 816, anno: 1999,
pagine: 165 - 174
SICI:
0006-8993(19990116)816:1<165:EOPDOS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALZHEIMERS-DISEASE; CEREBRAL-CORTEX; BINDING-SITES; RECEPTOR; SCHIZOPHRENIA; AGONIST; ANTAGONIST; ASSAY;
Keywords:
5-HT receptor; 5-HT1A receptor; adenylyl cyclase activity; rat and human brain postmortem;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Palego, L Univ6100a, Dept Psichiat Neurobiol Farmacol & Biotecnol, Via Bonanno 6, I-5 Univ Pisa Via Bonanno 6 Pisa Italy I-56100 , Via Bonanno 6, I-5
Citazione:
L. Palego et al., "Effects of postmortem delay on serotonin and (+)8-OH-DPAT-mediated inhibition of adenylyl cyclase activity in rat and human brain tissues", BRAIN RES, 816(1), 1999, pp. 165-174

Abstract

The reproducibility of serotonin (5-HT) and (+)8-OH-DPAT-mediated inhibition of adenylyl cyclase activity was assessed in membranes, stimulated by forskolin, of rat frontal cortex postmortem as well as of human fronto-cortical, hippocampal and dorsal raphe tissues obtained from autopsy brains. The results revealed that differences between basal and forskolin-stimulated enzyme activities were still significant after 48 h postmortem in rat cortex and in all human brain regions up to 46 h after death. However, a decrease of about 17 and 26% in forskolin-stimulated adenylyl cyclase activity was observed at 24 and 48 h, respectively, in rat cortex. 5-HT and the 5-HT1A receptor agonist, (+)8-hydroxy-2(di-N-propylamino)tetralin (8-OH-DPAT), were able to inhibit forskolin-stimulated adenylyl cyclase activity in a dose-dependent manner for 48 h after death in rat and human brain. In rat cortex, both 5-HT and (+)8-OH-DPAT potencies (EC50, nM) and efficacies (percent of maximum inhibition capacity, %) varied significantly with postmortem delay. Conversely, in human tissues, postmortem delay and subject age did not modify agonist potencies and efficacies. Furthermore, a regionality of 5-HT potency and efficacy was revealed in the human brain. 5-HT was equally potentin cortex and raphe nuclei, while being more potent but less effective in hippocampus. (+)8-OH-DPAT was more active in hippocampus and raphe nuclei than in cortex. (+)8-OH-DPAT behaved as an agonist in all areas, as its efficacy was similar or greater than those obtained with 5-HT. The (+)8-OH-DPATdose-response curve was completely reversed by 5-HT1A receptor antagonistsin rat cortex and all human brain areas. In conclusion, we suggest here that differences between rat and human brain might exist at the level of postmortem degradation of 5-HT-sensitive adenylyl cyclase activity. In human brain, 5-HT1A receptor-mediated inhibition of adenylyl cyclase seems to be reproducible, suggesting that reliable experiments can be carried out on postmortem specimens from patients with neuropsychiatric disorders. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 01:06:34