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Titolo:
Human growth factor-enhanced regeneration of transplantable human hematopoietic stem cells in nonobese diabetic/severe combined immunodeficient mice
Autore:
Cashman, JD; Eaves, CJ;
Indirizzi:
British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada British Columbia Canc Agcy Vancouver BC Canada V5Z 1L3 BC V5Z 1L3, Canada Univ British Columbia, Dept Med Genet, Vancouver, BC, Canada Univ British Columbia Vancouver BC Canada d Genet, Vancouver, BC, Canada
Titolo Testata:
BLOOD
fascicolo: 2, volume: 93, anno: 1999,
pagine: 481 - 487
SICI:
0006-4971(19990115)93:2<481:HGFROT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNE-DEFICIENT MICE; HUMAN CORD-BLOOD; EXPANSION IN-VITRO; ADULT BONE-MARROW; MULTILINEAGE HEMATOPOIESIS; RECONSTITUTING ABILITY; QUANTITATIVE ASSAY; VIVO; MAINTENANCE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Eaves, CJ BritishZColumbia Canc Agcy, Terry Fox Lab, 601 W 10th Ave, Vancouver, BC V5 British Columbia Canc Agcy 601 W 10th Ave Vancouver BC Canada V5Z 1L3
Citazione:
J.D. Cashman e C.J. Eaves, "Human growth factor-enhanced regeneration of transplantable human hematopoietic stem cells in nonobese diabetic/severe combined immunodeficient mice", BLOOD, 93(2), 1999, pp. 481-487

Abstract

Self-renewal is considered to be the essential defining property of a stemcell. Retroviral marking, in vitro amplification, and serial transplantation of human cells that can sustain long-term lymphomyelopoiesis in vivo have provided evidence that human hematopoietic stem cell self-renewal occurs both in vitro and in vivo. To investigate whether this process can be manipulated by cytokines, we administered two different combinations of human growth factors to sublethally irradiated nonobese diabetic/severe combined immunodeficient (SCID) mice transplanted with 10(7) light-density human cord blood cells and then performed secondary transplants to compare the number of transplantable human lymphomyeloid reconstituting cells present 4 to 6 weeks post-transplant. A 2-week course of Steel factor + interleukin (IL)-3 granulocyte-macrophage colony-stimulating factor + erythropoietin (3 times per week just before sacrifice) specifically and significantly enhanced the numbers of transplantable human lymphomyeloid stem cells detectable in the primary mice (by a factor of 10). Steel factor + Flt3-ligand + IL-6 (using either the same schedule or administered daily until sacrifice 4 weeks post-transplant) gave a threefold enhancement of this population. These effects were obtained at a time when the regenerating human progenitor populations in such primary mice are known to be maximally cycling even in the absence of growth factor administration suggesting that the underlying mechanism may reflect an ability of these growth factors to alter the probability of differentiation of stem cells stimulated to proliferate in vivo. (C) 1999by The American Society of Hematology.

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Documento generato il 03/07/20 alle ore 23:03:52