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Titolo:
A Mg2+-dependent endonuclease is responsible for internucleosomal DNA fragmentation in human B lymphoblastic IM9 cells
Autore:
Kwon, HJ; Kim, DS;
Indirizzi:
Yonsei Univ, Coll Sci, Dept Biochem, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 Biochem, Seoul 120749, South Korea Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 Res Ctr, Seoul 120749, South Korea
Titolo Testata:
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
fascicolo: 6, volume: 46, anno: 1998,
pagine: 1259 - 1268
SICI:
1039-9712(199812)46:6<1259:AMEIRF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-DEPENDENT ENDONUCLEASE; APOPTOTIC RAT THYMOCYTES; CA2+/MG2+-DEPENDENT ENDONUCLEASE; CLEAVAGE; IDENTIFICATION; PURIFICATION; ACTIVATION; PROTEIN;
Keywords:
apoptosis; Mg2+-dependent endonuclease; DNA-native-PAGE; IM9 cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, DS Yonsei Univ, Coll Sci, Dept Biochem, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 , Seoul 120749, South Korea
Citazione:
H.J. Kwon e D.S. Kim, "A Mg2+-dependent endonuclease is responsible for internucleosomal DNA fragmentation in human B lymphoblastic IM9 cells", BIOC MOL B, 46(6), 1998, pp. 1259-1268

Abstract

We have identified a Mg2+-dependent endonuclease activity from human B lymphoblastic IM9 cell lysates and nuclei using autodigestion method and DNA-native-polyacrylamide gel electrophoresis (DNA-native-PAGE) nuclease assay system. The level of the endonuclease activity in cell lysates was significantly decreased at certain stage by treatment of the cells with cycloheximide. However, the enzyme activity consistently remained for over 12 hours in the isolated nuclei of the apoptotic IM9 cells. The Mg2+-dependent endonuclease isolated from the nuclei by native-PAGE elution was able to catalyze the conversion of supercoiled plasmid DNA into linear form. This particular endonuclease activity was not detected in cycloheximide treated-U937 cells. Several lines of experimental evidence suggest that the Mg2+-dependent endonuclease localized in the nucleus may be responsible for the DNA fragmentation of apoptotic IM9 cells.

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Documento generato il 27/05/20 alle ore 02:28:55