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Titolo:
Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain
Autore:
Pedersen, H; Carlsen, M; Nielsen, J;
Indirizzi:
Technmark Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, De Tech Univ Denmark Lyngby Denmark DK-2800 Biotechnol, DK-2800 Lyngby, De
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 1, volume: 65, anno: 1999,
pagine: 11 - 19
SICI:
0099-2240(199901)65:1<11:IOEAQO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANNITOL CYCLE; SACCHAROMYCES-CEREVISIAE; PENICILLIUM-CHRYSOGENUM; PYRUVATE-CARBOXYLASE; CONTINUOUS CULTURES; NEUROSPORA-CRASSA; ALPHA-AMYLASE; NIDULANS; NIGER; NADPH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Pedersen, H TechLyngby,enmark, Dept Biotechnol, Ctr Proc Biotechnol, Bldg 223, DK-2800 Tech Univ Denmark Bldg 223 Lyngby Denmark DK-2800 23, DK-2800
Citazione:
H. Pedersen et al., "Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain", APPL ENVIR, 65(1), 1999, pp. 11-19

Abstract

Two alpha-amylase-producing strains of Aspergillus oryzae, a wild-type strain and a recombinant containing additional copies of the alpha-amylase gene, were characterized,vith respect to enzyme activities, localization of enzymes to the mitochondria or cytosol, macromolecular composition, and metabolic fluxes through the central metabolism during glucose-limited chemostatcultivations. Citrate synthase and isocitrate dehydrogenase (NAD) activities were found only in the mitochondria, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase (NADP) activities were found only in the cytosol, and isocitrate dehydrogenase (NADP), glutamate oxaloacetate transaminase,malate dehydrogenase, and glutamate dehydrogenase (NAD) activities were found in both the mitochondria and the cytosol, The measured biomass components and ash could account for 95% (wt/wt) of the biomass. The protein and RNA contents increased linearly with increasing specific growth rate, but thecarbohydrate and chitin contents decreased. A metabolic model consisting of 69 fluxes and 59 intracellular metabolites was used to calculate the metabolic fluxes through the central metabolism at several specific growth rates, with ammonia or nitrate as the nitrogen source. The flux through the pentose phosphate pathway increased with increasing specific growth rate. The fluxes through the pentose phosphate pathway were 15 to 26% higher for the recombinant strain than for the wild-type strain.

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Documento generato il 14/07/20 alle ore 10:12:48