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Titolo:
Identification of phosphoenolpyruvate carboxylase isoforms in leaf, stem and roots of the obligate CAM plant Vanilla planifolia Salib. (Orchidaceae):a physiological and molecular approach
Autore:
Gehrig, H; Faist, K; Kluge, M;
Indirizzi:
Tech Univ Darmstadt, Inst Bot, D-64287 Darmstadt, Germany Tech Univ Darmstadt Darmstadt Germany D-64287 D-64287 Darmstadt, Germany
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 6, volume: 38, anno: 1998,
pagine: 1215 - 1223
SICI:
0167-4412(199812)38:6<1215:IOPCII>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-3 PLANTS; METABOLISM; EXPRESSION; STRESS; GENES;
Keywords:
crassulacean acid metabolism (CAM); Orchidaceae; phoshoenolpyruvate carboxylase; isoforms; gas exchange; Vanilla planifolia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Gehrig, H Technyniv Darmstadt, Inst Bot, Schnittspahnstr 10, D-64287 Darmstadt, Germa Tech Univ Darmstadt Schnittspahnstr 10 Darmstadt Germany D-64287
Citazione:
H. Gehrig et al., "Identification of phosphoenolpyruvate carboxylase isoforms in leaf, stem and roots of the obligate CAM plant Vanilla planifolia Salib. (Orchidaceae):a physiological and molecular approach", PLANT MOL B, 38(6), 1998, pp. 1215-1223

Abstract

This study provides the first comparative analysis of phosphoenolpyruvate carboxylase isoforms (PEPc; EC 4.1.1.31) in an obligate crassulacean acid metabolism (CAM) plant, Vanilla planifolia Salisb. (Orchidaceae). Nocturnal CO2 fixation and malate accumulation by the leaves and the green stem show that these organs perform CAM. The chloroplast-containing aerial roots, however, exhibit C3 photosynthesis. The catalytic activity of PEPc was highestin the leaves compared with the stem and aerial roots. The K-m (PEP) and K-i (malate) were similar in the PEPc extracted from leaf and aerial roots, and significant higher in stem, cDNA was obtained from those tissues and also from the soil-grown roots, and various cDNA clones were detected and amplified by means of RT-PCR and RACE-PCR. The amino-acid sequences of the PEPc isoforms deduced from the cDNA showed a great degree of homology, and Southern blot analysis suggests that the encoding genes form a small multigenefamily of at least two members. One PEPc isoform (PpcV1) is assumed to be related to CAM because, as shown by northern blot analysis, it is mainly expressed in the CAM-performing organs, i.e. in the leaves and the stern. A further isoform (PpcV2) was identified in the soil-grown roots and aerial roots, but northern blots show that to some extent PpcV2 is also expressed inthe leaf and the stem tissues. Thus, it is assumed that PpcV2 encodes the housekeeping isoform of PEPc. Altogether, the present study provides support in favour of the view that isoforms of PEPc are related to specific functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:55:26