Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A novel In-111-labeled antisense DNA probe with multi-chelating sites (MCSprobe) showing high specific radioactivity and labeling efficiency
Autore:
Fujibayashi, Y; Yoshimi, E; Waki, A; Sakahara, H; Saga, T; Konishi, J; Yonekura, Y; Yokoyama, A;
Indirizzi:
Kyoto Univ, Grad Sch Pharmaceut Sci, Sakyo Ku, Kyoto 606, Japan Kyoto Univ Kyoto Japan 606 ch Pharmaceut Sci, Sakyo Ku, Kyoto 606, Japan Kyoto Univ, Grad Sch Med, Sakyo Ku, Kyoto 606, Japan Kyoto Univ Kyoto Japan 606 niv, Grad Sch Med, Sakyo Ku, Kyoto 606, Japan Fukui Med Univ, Biomed Imaging Res Ctr, Fukui 91011, Japan Fukui Med UnivFukui Japan 91011 med Imaging Res Ctr, Fukui 91011, Japan
Titolo Testata:
NUCLEAR MEDICINE AND BIOLOGY
fascicolo: 1, volume: 26, anno: 1999,
pagine: 17 - 21
SICI:
0969-8051(199901)26:1<17:ANIADP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEIC-ACIDS; DIGOXIGENIN SYSTEM; OLIGONUCLEOTIDES;
Keywords:
antisense; oncogene-mRNA; indium-111; high specific radioactivity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Fujibayashi, Y Kyoto Univ, Grad Sch Pharmaceut Sci, Sakyo Ku, Kyoto 606, Japan Kyoto Univ Kyoto Japan 606 ci, Sakyo Ku, Kyoto 606, Japan
Citazione:
Y. Fujibayashi et al., "A novel In-111-labeled antisense DNA probe with multi-chelating sites (MCSprobe) showing high specific radioactivity and labeling efficiency", NUCL MED BI, 26(1), 1999, pp. 17-21

Abstract

A multi-aminolinked oligodeoxynucleotide (ODN) was synthesized by substitution of dT with aminolinked dU in the sequence, following conjugation with isothiocyanobenzyl-EDTA (IBE) for In-111 labeling. As a model target gene, the c-erbB-2 protooncogene was used. The probability of the number of aminolinked dU in the 20mer ODN was 5, but there were actually 3 and 4 in the selected antisense and sense ODNs, respectively. The IBE/ODN conjugation levels of probes with multi chelating sites (MCS-probe) were 1.6 (antisense) and 2.4 (sense), more than 50 times higher than those of our previous studiesusing 5'-end aminolinked ODNs (IBE/ODN = 0.03), Labeling studies using theMCS probe and In-111 indicated that specific radioactivity as high as 48 MBq/nmol could be obtained with a labeling efficiency of over 90%. The In-111 antisense MCS-probe could bound to sense ODN under physiological conditions, but the In-111-sense-MCA-probe could not. Thus, side-chain modificationof ODN for metal labeling is considered to be useful for antisense techniques. NUCL MED BIOL 26;1:17-21, 1999. (C) 1998 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:28:23