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Titolo:
Quantitation of 5HT(2A) receptor mRNA in human postmortem brain using competitive RT-PCR
Autore:
Dwivedi, Y; Pandey, GN;
Indirizzi:
Univ Illinois, Coll Med, Dept Psychiat, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 , Dept Psychiat, Chicago, IL 60612 USA
Titolo Testata:
NEUROREPORT
fascicolo: 17, volume: 9, anno: 1998,
pagine: 3761 - 3765
SICI:
0959-4965(199812)9:17<3761:QO5RMI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MESSENGER-RNAS; SUICIDE VICTIMS; 5-HT2 RECEPTOR; BINDING-SITES; SEROTONIN; 1A;
Keywords:
competitive RT-PCR; 5HT(2A) receptor; mRNA; human postmortem brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Pandey, GN Univ Illinois, Coll Med, Dept Psychiat, 1601 W Taylor St, Chicago, IL 60612 Univ Illinois 1601 W Taylor St Chicago IL USA 60612 o, IL 60612
Citazione:
Y. Dwivedi e G.N. Pandey, "Quantitation of 5HT(2A) receptor mRNA in human postmortem brain using competitive RT-PCR", NEUROREPORT, 9(17), 1998, pp. 3761-3765

Abstract

WE describe quantitation of the absolute amounts of 5HT(2A) receptor mRNA in various areas of human postmortem brain using a very sensitive and specific technique: competitive reverse transcription polymerase chain reaction (RT-PCR). With this procedure, we were able to show that the highest levelsof 5HT(2A) receptor mRNA are in cerebral cortical areas, i.e., Brodmann's areas 8, 9, 10, 18, 19, 22, 24, and 46, and that intermediate levels occur in the hippocampus, the amygdala, the nucleus accumbens, and the hypothalamus. Very low levels of 5HT(2A) receptor mRNA were found in the cerebellum, the thalamus, the caudate, and the putamen. Our results confirm the heterogeneous distribution of 5HT(2A) receptor mRNA in human postmortem brain and suggest that competitive RT-PCR is a highly sensitive and specific procedure for the quantitative measurement of 5HT(2A) receptor mRNA expression. Thestudy of 5HT(2A) receptor mRNA in different brain areas will help clarify the regulation of 5HT(2A) receptors at the transcriptional level in variousneuropsychiatric diseases. NeuroReport 9: 3761-3765 (C) 1998 Lippincott Williams & Wilkins.

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Documento generato il 25/11/20 alle ore 18:29:26