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Titolo:
THE EFFECTS OF CA IONS, EGTA AND STORAGE TIME ON MYOFIBRILLAR PROTEIN-DEGRADATION, LEVELS OF CA2-DEPENDENT PROTEASES AND CATHEPSIN-B, CATHEPSIN-H, CATHEPSIN-L AND CATHEPSIN-D OF OSTRICH SKELETAL-MUSCLE()
Autore:
VANJAARSVELD FP; NAUDE RJ; OELOFSEN W;
Indirizzi:
UNIV PORT ELIZABETH,DEPT BIOCHEM,POB 1600 ZA-6000 PORT ELIZABETH SOUTH AFRICA UNIV PORT ELIZABETH,DEPT BIOCHEM ZA-6000 PORT ELIZABETH SOUTH AFRICA
Titolo Testata:
Meat science
fascicolo: 4, volume: 45, anno: 1997,
pagine: 517 - 529
SICI:
0309-1740(1997)45:4<517:TEOCIE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-ACTIVATED FACTOR; LYSOSOMAL CYSTEINE PROTEINASE; POSTMORTEM TENDERIZATION; DEPENDENT PROTEASES; OVINE CARCASSES; CHICKEN MUSCLE; BOVINE; CALPAIN; PH; FRAGMENTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
F.P. Vanjaarsveld et al., "THE EFFECTS OF CA IONS, EGTA AND STORAGE TIME ON MYOFIBRILLAR PROTEIN-DEGRADATION, LEVELS OF CA2-DEPENDENT PROTEASES AND CATHEPSIN-B, CATHEPSIN-H, CATHEPSIN-L AND CATHEPSIN-D OF OSTRICH SKELETAL-MUSCLE()", Meat science, 45(4), 1997, pp. 517-529

Abstract

The effect of Ca ions and ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) on myofibrillar protein degradation showed that when ostrich iliotibialis lateralis muscle was incubated with 10 mM EGTA at 2-4 degrees C for 24 hr, the activity of extracted cathepsin H was unchanged compared with a buffer-incubated sample. Ca+had no effect on extracted cathepsin H activity, while that of Ca2+-dependent protease (CDP) decreased significantly (p < 0.05). Ca2+-treatment enhanced post-mortem changes observed in myofibrillar protein patterns (production of fragments around 30 K) that were not observed in EGTA-incubated myofibrils. The effect of storage time on shear force, CDP activity, cathepsin B, D, H and L activities and the SDS-PAGE pattern of myofibrils showed a time-dependent reduction in CDP activity. Of the cathepsins studied only cathepsin H showed a reduction (40%) in activity. The most prominent component appearing on storage at 2-4 degrees C had a M(r) of 27 K. The incubation of myofibrils with CDP mimicked the post-mortem changes. CDP may be responsible for some of the post-mortem changes observed, although shear force measurements suggest these changes do not lead to significant tenderisation. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:37:53