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Titolo:
Electron microscopy and X-ray diffraction studies of Lotus tetragonolobus A isolectin cross-linked with a divalent Lewis(x) oligosaccharide, an oncofetal antigen
Autore:
Cheng, W; Bullitt, E; Bhattacharyya, L; Brewer, CF; Makowski, L;
Indirizzi:
YeshivaUSAiv Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 Bronx, NY 10461 Yeshiva Univ Albert Einstein Coll Med, Dept Microbiol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Immunol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Boston Univ, Dept Phys, Boston, MA 02215 USA Boston Univ Boston MA USA 02215 ton Univ, Dept Phys, Boston, MA 02215 USA Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA Florida State Univ Tallahassee FL USA 32306 ys, Tallahassee, FL 32306 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 52, volume: 273, anno: 1998,
pagine: 35016 - 35022
SICI:
0021-9258(199812)273:52<35016:EMAXDS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYSTAL-STRUCTURE; CONCANAVALIN-A; 3-DIMENSIONAL STRUCTURE; QUATERNARY STRUCTURE; SOYBEAN AGGLUTININ; ANIMAL LECTINS; LEGUME LECTIN; BINDING; COMPLEX; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Brewer, CF Yeshivave,iv Albert Einstein Coll Med, Dept Mol Pharmacol, 1300Morris Pk A Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave BronxNY USA 10461
Citazione:
W. Cheng et al., "Electron microscopy and X-ray diffraction studies of Lotus tetragonolobus A isolectin cross-linked with a divalent Lewis(x) oligosaccharide, an oncofetal antigen", J BIOL CHEM, 273(52), 1998, pp. 35016-35022

Abstract

The interactions of lectins with multivalent carbohydrates often leads to the formation of highly ordered cross-linked lattices that are amenable to structural studies. A particularly well ordered, two-dimensional lattice isformed from fucose-specific isolectin A from Lotus tetragonolobus cross-linked with difucosyllacto-N-neohexaose, an oligosaccharide possessing the Lewis(x) determinant, which is an oncofetal antigen. A combination of electron microscopy, x-ray diffraction, simulation of electron micrographs, and molecular model building was used to determine the relative positions of the tetrameric lectin and bivalent carbohydrate within the lattice. X-ray diffraction from unoriented pellets was used to determine the lattice dimensionsand analysis of electron micrographs was used to determine the lattice symmetry. Molecular models of the lattice were constructed based on the known structure of the jack bean lectin concanavalin A and the high degree of sequence homology between the two lectins. Using the symmetry and dimensions of the lattice and its appearance in filtered electron micrographs, molecular models were used to determine the orientation of the lectin in the lattice, and to define the range of lectin-oligosaccharide interactions consistent with the structural data. The present study provides the first description of a highly ordered, two-dimensional, cross-linked lattice between a tetravalent lectin and a bivalent carbohydrate.

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Documento generato il 01/12/20 alle ore 10:56:18