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Titolo:
SODIUM-BUTYRATE STIMULATION OF HIV-1 GENE-EXPRESSION - A NOVEL MECHANISM OF INDUCTION INDEPENDENT OF NF-KAPPA-B
Autore:
LAUGHLIN MA; CHANG GY; OAKES JW; GONZALEZSCARANO F; POMERANTZ RJ;
Indirizzi:
THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,DEPT MED,DIV INFECT DIS,DORRANCE H HAMILTON LABS PHILADELPHIA PA 19107 UNIV PENN,MED CTR,DEPT NEUROL PHILADELPHIA PA 19104 UNIV PENN,MED CTR,DEPT MICROBIOL PHILADELPHIA PA 19104
Titolo Testata:
Journal of acquired immune deficiency syndromes and human retrovirology
fascicolo: 4, volume: 9, anno: 1995,
pagine: 332 - 339
SICI:
1077-9450(1995)9:4<332:SSOHG->2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; SCAFFOLD-ATTACHED REGIONS; NUCLEOSOME CORE PARTICLE; TOPOISOMERASE-II SITES; LONG TERMINAL REPEAT; TISSUE-SPECIFIC GENE; BETA-GLOBIN GENE; HISTONE ACETYLATION; TRANSGENIC MICE; TRANSCRIPTION FACTOR;
Keywords:
SODIUM BUTYRATE; CHROMATIN STRUCTURE; HIV-1 LATENCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Laughlin et al., "SODIUM-BUTYRATE STIMULATION OF HIV-1 GENE-EXPRESSION - A NOVEL MECHANISM OF INDUCTION INDEPENDENT OF NF-KAPPA-B", Journal of acquired immune deficiency syndromes and human retrovirology, 9(4), 1995, pp. 332-339

Abstract

Nuclear factor-kappa B (NF-kappa B) has been shown to play a central role in stimulating human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR)-directed viral gene expression. We have previously described a cell line (TE671/RD) that fails to respond to phorbol myristate acetate (PMA) or tumor necrosis factor-alpha (TNF-alpha) in terms of amplifying HIV-1 LTR-driven gene expression unless it is concurrently treated with sodium butyrate. It was not determined whether this lack of response stemmed from an inability of these cells to producefree NF-kappa B or from ineffectual interaction of this sequence-specific transcriptional factor with its target. We now show that these cells are in fact capable of inducing a free nuclear NF-kappa B-binding activity when stimulated with PMA but not when treated with sodium butyrate alone. Furthermore, we show that sodium butyrate alone is equally potent in stimulating HIV-1 LTR-directed gene expression in latentlyinfected U1 and ACH-2 cells in the absence of induction of nuclear NF-kappa B, as compared with PMA, which induces NF-kappa B activation inthese cells. We also show that stimulation of HIV-1 expression in U1 cells with sodium butyrate is not blocked by N-acetylcysteine, whereasthat of PMA stimulation is blocked. These observations are discussed in the context of a model where chromatin structure participates in the maintenance of restricted HIV-1 viral gene expression in these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 23:48:09