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Titolo:
Enhancement of 5-fluorouracil cytotoxicity by human thymidine-phosphorylase expression in cancer cells: In vitro and in vivo study
Autore:
Evrard, A; Cuq, P; Robert, B; Vian, L; Pelegrin, A; Cano, JP;
Indirizzi:
Univ Montpellier 1, Fac Pharm, F-34060 Montpellier 02, France Univ Montpellier 1 Montpellier France 02 F-34060 Montpellier 02, France Ctr Rech Cancerol Val Aurelle Paul Lamarque, Montpellier, France Ctr Rech Cancerol Val Aurelle Paul Lamarque Montpellier France , France
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 3, volume: 80, anno: 1999,
pagine: 465 - 470
SICI:
0020-7136(19990129)80:3<465:EO5CBH>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLON-CARCINOMA CELLS; GROWTH-FACTOR; BLOOD-PLATELETS; NORMAL-TISSUES; KINASE GENES; SENSITIVITY; PURIFICATION; TUMORS; INTERFERON; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Cuq, P Univ Montpellier 1, Fac Pharm, 15 Ave Charles Flahault, F-34060 Montpellier Univ Montpellier 1 15 Ave Charles Flahault Montpellier France 02 r
Citazione:
A. Evrard et al., "Enhancement of 5-fluorouracil cytotoxicity by human thymidine-phosphorylase expression in cancer cells: In vitro and in vivo study", INT J CANC, 80(3), 1999, pp. 465-470

Abstract

Transferring a gene into cancer cells in order to sensitize them to drugs is an important approach in human cancer gene-therapy research. Thymidine phosphorylase (TP) is the first enzyme in the metabolic activation pathway of 5-fluorouracil (5-FU) to fluorodeoxyribonucleotides, thus, it could be used to increase the sensitivity of cancer cells to this anti-pyrimidine agent. In this study, an expression vector containing the human TP cDNA was transfected into C26 murine colon-carcinoma cells. Stable transfectants were selected; all showed increased TP activity, ranging from 2- to 10-fold when compared with wild-type cells. The in vitro sensitivity of transfectants to5-FU and 5'-deoxy-5-fluorouridine (5'-DFUR) was enhanced, in agreement with the observed increase in TP activity. Then, tumors were generated by s.c.injection of TP-transfected or wild-type C26 cells in syngeneic BALB/c mice. 5-FU (25 mg/kg, i.p.) induced a growth delay of TP-transfected C26 tumors as compared with C26 wild-type tumors. These data suggest that TP could be transfected in tumor cells to increase the sensitivity to 5-FU for subsequent cancer gene therapy. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 08:13:02