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Titolo:
Partial characterization and isolation of earwig 'allatostatins': biological activities in earwigs and cockroaches
Autore:
Rankin, SM; Garside, CS; Christopher, CA; Tobe, SS;
Indirizzi:
Allegheny Coll, Dept Biol, Meadville, PA 16335 USA Allegheny Coll Meadville PA USA 16335 Dept Biol, Meadville, PA 16335 USA Univ Toronto, Dept Zool, Toronto, ON M5S 3G5, Canada Univ Toronto TorontoON Canada M5S 3G5 Zool, Toronto, ON M5S 3G5, Canada
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 4, volume: 121, anno: 1998,
pagine: 395 - 403
SICI:
1095-6433(199812)121:4<395:PCAIOE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
JUVENILE-HORMONE BIOSYNTHESIS; BLOWFLY CALLIPHORA-VOMITORIA; DIPLOPTERA-PUNCTATA; CORPORA-ALLATA; FUNCTIONAL-SIGNIFICANCE; SCHISTOCERCA-GREGARIA; DEVELOPMENTAL STAGES; GRYLLUS-BIMACULATUS; IDENTIFICATION; NEUROPEPTIDES;
Keywords:
allatostatin; radioimmunoassay; corpora allata; juvenile hormone production; proctolin; hindgut contractions; earwigs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Rankin, SM Allegheny Coll, Dept Biol, Meadville, PA 16335 USA Allegheny Coll Meadville PA USA 16335 Meadville, PA 16335 USA
Citazione:
S.M. Rankin et al., "Partial characterization and isolation of earwig 'allatostatins': biological activities in earwigs and cockroaches", COMP BIOC A, 121(4), 1998, pp. 395-403

Abstract

Allatostatins are a family of neuropeptides first isolated from the cockroach, Diploptera punctata, that inhibit juvenile hormone production in that species (but do not do so in earwigs), and inhibit hindgut muscle contractions in some insects, including the earwig, Euborellia annulipes. We examined whether material from earwig brains is similar to cockroach allatostatinsbiochemically, immunologically and physiologically. Brain extracts from adult female earwigs were separated by high performance liquid chromatography(HPLC), followed by radioimmunoassay using antibodies to cockroach allatostatin (Dip-AST). Fractions that co-eluted with cockroach allatostatins wereimmunoreactive, and at least two peaks of immunoreactivity were detected. Material from each peak at 10 nM Dip-AST equivalents inhibited juvenile hormone biosynthesis in vitro by corpora allata of 2-day virgin D. punctata cockroaches; 1 nM was less effective, and non-immunoreactive fractions failedto inhibit juvenile hormone biosynthesis. Both crude and Sep-Pak (Waters) purified extracts of brains of earwigs containing 1 nM Dip-AST equivalents failed to suppress hindgut contractions in vitro of 2-day earwigs and of brooding female earwigs. In contrast, 1 nM cockroach allostatin (Dip-AST 7) reversibly inhibited hindgut contractions in vitro. These results suggested the: presence of another brain factor, such as proctolin, that counteracts the inhibitory effects of Dip-AST. In support of this hypothesis, proctolinstimulated hindgut contractions in vitro at I nM, the effects of equal concentrations of allatostatin and proctolin varied with the stage of the female. Furthermore, HPLC-separated fractions that co-eluted with cockroach allatostatin and were immunoreactive with antibodies to Dip-AST suppressed hindgut contractions in vitro of 3-day female earwigs. Finally, crude brain extracts of earwigs suppressed earwig juvenile hormone biosynthesis in vitro in glands of low, but not in glands of high, activity. Thus, earwig blain extract after HPLC separation has Dip-AST-like material that inhibits cockroach corpora allata and suppresses earwig hindgut contractions. Sep-Pak-extracted earwig brain material, however, does not inhibit earwig gut contraction. Although synthetic Dip-AST 7 does not inhibit juvenile hormone synthesis by earwig corpora allata, there is heat-stable material in earwig brain extract that does have this action. (C) 1998 Elsevier Science Inc. All rights reserved.

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Documento generato il 04/07/20 alle ore 12:07:51