Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Heparin and high density lipoprotein mediate bovine sperm capacitation by different mechanisms
Autore:
Lane, ME; Therien, I; Moreau, R; Manjunath, P;
Indirizzi:
Hopdaaison Neuve Rosemont, Ctr Rech Guy Bernier, Montreal, PQ H1T 2M4, Cana Hop Maison Neuve Rosemont Montreal PQ Canada H1T 2M4 al, PQ H1T 2M4, Cana Univ Montreal, Dept Biochem, Montreal, PQ H1T 2M4, Canada Univ Montreal Montreal PQ Canada H1T 2M4 em, Montreal, PQ H1T 2M4, Canada Univ Montreal, Dept Med, Montreal, PQ H1T 2M4, Canada Univ Montreal Montreal PQ Canada H1T 2M4 ed, Montreal, PQ H1T 2M4, Canada
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 1, volume: 60, anno: 1999,
pagine: 169 - 175
SICI:
0006-3363(199901)60:1<169:HAHDLM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE PHOSPHORYLATION; SEMINAL PLASMA; ACROSOME REACTION; MAJOR PROTEINS; BINDING PROTEINS; CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE; BIOCHEMICAL-CHARACTERIZATION; INVITRO CAPACITATION; SPERMATOZOA INVITRO; HAMSTER SPERMATOZOA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Manjunath, P Hopl,aison Neuve Rosemont, Ctr Rech Guy Bernier, 5415 Blvd Assompt, Montrea Hop Maison Neuve Rosemont 5415 Blvd Assompt Montreal PQ Canada H1T 2M4
Citazione:
M.E. Lane et al., "Heparin and high density lipoprotein mediate bovine sperm capacitation by different mechanisms", BIOL REPROD, 60(1), 1999, pp. 169-175

Abstract

Capacitation is an important process in bovine sperm maturation and is an obligatory step prior to fertilization. Two capacitating agents, namely heparin and high-density lipoprotein (HDL), have been shown to induce sperm capacitation. A family of major proteins of bovine seminal plasma designated BSP-A1/A2, BSP-A3, and BSP-30 kDa (collectively called BSP proteins) bind to the sperm surface upon ejaculation via their membrane choline phospholipids. Our previous studies with bovine epididymal sperm showed that BSP proteins potentiate sperm capacitation induced by heparin and HDL. This study was undertaken to clarify the mechanism of capacitation induced by heparin and HDL in the presence of BSP proteins. Washed bovine ejaculated sperm were incubated with heparin (12 mu g/ml) or HDL (10-160 mu g/ml) in the presenceof polyclonal antibodies against purified BSP proteins (anti-BSP proteins). The percentage of capacitated sperm was evaluated after the induction of the acrosome reaction (AR) with lysophosphatidylcholine. When sperm were incubated for 5 h with heparin and anti-BSP proteins (40 mu g/ml), the AR level was not significantly different from control levels (16.8 +/- 0.9% vs. 12.9 +/- 0.9%). In contrast, incubation of sperm for 8 h with HDL and anti-BSP proteins did not inhibit the AR (42.4 +/- 1.1% vs. 17.1 +/- 1.6 for the control samples). We also investigated the effect of heparin and HDL on protein tyrosine phosphorylation associated with capacitation. The tyrosine phosphorylation of a group of proteins was increased in the presence of heparin. However, HDL did not significantly stimulate protein phosphorylation. The increase in phosphorylation was correlated with an increase in the AR after the incubation with heparin but not:vith HDL. These results indicate that heparin and HDL mediate capacitation via different mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:50:46