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Titolo:
Parasitological detection of Trypanosoma brucei gambiense in serologicallynegative sleeping-sickness suspects from north-western Uganda
Autore:
Enyaru, JCK; Matovu, E; Akol, M; Sebikali, C; Kyambadde, J; Schmidt, C; Brun, R; Kaminsky, R; Ogwal, LM; Kansiime, F;
Indirizzi:
Livestock Hlth Res Inst, Tororo, Uganda Livestock Hlth Res Inst Tororo Uganda ock Hlth Res Inst, Tororo, Uganda Makerere Univ, Dept Biochem, Kampala, Uganda Makerere Univ Kampala Uganda kerere Univ, Dept Biochem, Kampala, Uganda Swiss Trop Inst, CH-4002 Basel, Switzerland Swiss Trop Inst Basel Switzerland CH-4002 st, CH-4002 Basel, Switzerland Coordinating Off Control Trypanosomiasis Uganda, Entebbe, Uganda Coordinating Off Control Trypanosomiasis Uganda Entebbe Uganda , Uganda
Titolo Testata:
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY
fascicolo: 8, volume: 92, anno: 1998,
pagine: 845 - 850
SICI:
0003-4983(199812)92:8<845:PDOTBG>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE GLYCOPROTEIN GENES; AMPLIFICATION; DIAGNOSIS; CATT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Enyaru, JCK Livestock Hlth Res Inst, POB 96, Tororo, Uganda Livestock HlthRes Inst POB 96 Tororo Uganda Tororo, Uganda
Citazione:
J.C.K. Enyaru et al., "Parasitological detection of Trypanosoma brucei gambiense in serologicallynegative sleeping-sickness suspects from north-western Uganda", ANN TROP M, 92(8), 1998, pp. 845-850

Abstract

Forty-five parasitologically confirmed cases of sleeping sickness were diagnosed in north-western Uganda using a combination of two or three techniques. Forty of the cases were positive by the card agglutination test for trypanosomiasis (CATT), four were negative and one was not screened by the CATT. Trypanosomes isolated from the four CATT-negative but parasitologically positive cases were propagated for detailed biochemical genetic analysis. The aim was to demonstrate whether these four stocks lacked the LiTat 1.3 gene which encodes the antigen on which the CATT is based. All the DNA extracts isolated from these CATT-negative stocks and from six CATT-positive stocks of Trypanosoma brucei gambiense were targeted for amplification by the three variable-surface-glycoprotein genes thought to be ubiquitous in T. b. gambiense. The LiTat 1.3 gene was shown to be present in all 10 stocks. Trypanosome carriers may be CATT-negative because the CATT is not sensitive enough, because their parasites lack the LiTat 1.3 gene, or because their parasites have this gene but do not express it. The four sleeping-sickness cases who gave negative CATT results in the present study have very important implications in the diagnosis of T. b. gambiense infections using the CATT. Following treatment of the CATT-positive cases, the CATT-negative carriersof the trypanosomes remain as human reservoir hosts for continuous infection of the population. Because CATT-negative individuals are rarely examinedfurther, the general prevalence of parasitologically positive but CATT-negative cases is unclear. This study demonstrates the value of co-ordinated use of serological and parasitological techniques in the diagnosis of Gambian sleeping sickness.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:03:40