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Titolo:
Regulation of sodium transport in M-1 cells
Autore:
Nakhoul, NL; Hering-Smith, KS; Gambala, CT; Hamm, LL;
Indirizzi:
TulaneUSAiv, Med Ctr, Nephrol Sect, Sch Med,Dep Med, New Orleans, LA 70112Tulane Univ New Orleans LA USA 70112 h Med,Dep Med, New Orleans, LA 70112 Tulane Univ, Sch Med, Dept Physiol, New Orleans, LA 70112 USA Tulane UnivNew Orleans LA USA 70112 t Physiol, New Orleans, LA 70112 USA Vet Affairs Med Ctr, New Orleans, LA 70112 USA Vet Affairs Med Ctr New Orleans LA USA 70112 r, New Orleans, LA 70112 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 6, volume: 44, anno: 1998,
pagine: F998 - F1007
SICI:
0363-6127(199812)44:6<F998:ROSTIM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING DUCT; NONSELECTIVE CATION CHANNEL; EPIDERMAL GROWTH-FACTOR; SENSITIVE NA+ CHANNEL; RAT CCD; MINERALOCORTICOID HORMONES; POTASSIUM-TRANSPORT; WATER TRANSPORT; TUBULE; VASOPRESSIN;
Keywords:
aldosterone; dexamethasone; arginine vasopressin; aprotinin; collecting duct cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Hamm, LL TulaneNewiv, Med Ctr, Nephrol Sect, Sch Med,Dep Med, SL45,1430 Tulane Ave, Tulane Univ SL45,1430 Tulane Ave New Orleans LA USA 70112 ne Ave,
Citazione:
N.L. Nakhoul et al., "Regulation of sodium transport in M-1 cells", AM J P-REN, 44(6), 1998, pp. F998-F1007

Abstract

The M-1 cell line, derived from the mouse cortical collecting duct (CCD), is being used as a mammalian model of the CCD to study Na+ transport. The present studies aimed to further define the role of various hormones in affecting Na+ transport in M-1 cells grown in defined media. M-1 cells on permeable support, in serum-free media, developed amiloride-sensitive current 4-5 days after seeding. As expected for the involvement of epithelial Na+ channels, alpha-, beta-, and gamma-subunits of the epithelial Na+ channel wereidentified by RT-PCR. Either dexamethasone (Dex, 10-100 nM) or aldosterone(Aldo, 10(-6)-10(-7) M) for 24 h stimulated transport. Cells grown in the presence of Aldo and Dex had higher transport than with Dex alone. Spironolactone added to Dex media decreased transport. The acute effects of hormones reported to inhibit Na+ transport in CCD were also examined. Epidermal growth factor, phorbol esters, and increased intracellular Ca2+ with thapsigargin did not alter transport. Arginine vasopressin caused a transient increase in transport (probably Cl- secretion), which was not amiloride sensitive. Also, the protease inhibitor aprotinin decreased Na+ transport; in aprotinin-treated cells, trypsin stimulated transport. This study demonstrates that adrenal steroids (Dex > Aldo) stimulate Na+ transport in M-1 cells. At least part of this response may represent activation of mineralocorticoid receptors based on an additive effect of Dex and Aldo, as well as inhibitionby spironolactone. Responses to immediate-acting hormones is limited. However, an endogenous protease activity, which activates Na+ transport, is present in these cells.

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Documento generato il 21/09/20 alle ore 11:13:00