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Titolo:
Upregulation of H+-ATPase in the distal nephron during potassium depletion: structural and functional evidence
Autore:
Bailey, MA; Fletcher, RM; Woodrow, DF; Unwin, RJ; Walter, SJ;
Indirizzi:
UnivEngland Univ Coll, Ctr Nephrol, Dept Med, Rayne Inst, London WC1E 6JJ,Univ London Univ Coll London England WC1E 6JJ yne Inst, London WC1E 6JJ, Charing Cross Hosp, Imperial Coll Sch Med, Dept Histopathol, Div Investigat Charing Cross Hosp London England W6 8RF ept Histopathol, Div Investigat Charingngland Hosp, Imperial Coll Sch Med, Div Biomed Sci, London W6 8RF, E Charing Cross Hosp London England W6 8RF iv Biomed Sci, London W6 8RF, E
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 6, volume: 44, anno: 1998,
pagine: F878 - F884
SICI:
0363-6127(199812)44:6<F878:UOHITD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDULLARY COLLECTING DUCT; TRANSLOCATING ADENOSINE-TRIPHOSPHATASE; RENAL BICARBONATE REABSORPTION; BOVINE KIDNEY MEDULLA; RAT-KIDNEY; INTERCALATED CELLS; CARBONIC-ANHYDRASE; TUBULE; ACIDIFICATION; TRANSPORT;
Keywords:
proton-adenosinetriphosphatase; bafilomycin A(1); transepithelial potential difference; cortical collecting duct;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Bailey, MA Univ London Univ Coll, Ctr Nephrol, Dept Med, Rayne Inst, 5 Univ St, London Univ London Univ Coll 5 Univ St London England WC1E 6JJ London
Citazione:
M.A. Bailey et al., "Upregulation of H+-ATPase in the distal nephron during potassium depletion: structural and functional evidence", AM J P-REN, 44(6), 1998, pp. F878-F884

Abstract

In the present study, we have investigated the effects of dietary potassium depletion on the activity and distribution of the H+-ATPase in the distalnephron of the Sprague-Dawley rat. H+-ATPase activity was assessed from the change in transepithelial potential difference (V-te) in response to bafilomycin A(1) during perfusion of the late distal tubule in vivo, with solutions containing inhibitors of known ion channels. Bafilomycin A(1) caused anegative deflection in V-te in control animals, an effect that was significantly enhanced during potassium depletion (P < 0.01). The distribution of H+-ATPase within the population of intercalated cells was assessed using a specific monoclonal antibody (E11). Hypokalemia was associated with a highly significant redistribution of the staining pattern (P < 0.001), with an increase in the percentage of cells displaying immunoreactivity in the apical membrane. These results indicate that dietary potassium depletion increases electrogenic H+-ATPase activity in the rat distal tubule; this may be associated with increased insertion of pumps into the apical membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 18:29:31