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Titolo:
Expression of TWIK-1, a novel weakly inward rectifying potassium channel in rat kidney
Autore:
Cluzeaud, F; Reyes, R; Escoubet, B; Fay, M; Lazdunski, M; Bonvalet, JP; Lesage, F; Farman, N;
Indirizzi:
Fac Med Xavier Bichat, INSERM, U478, F-75870 Paris 18, France Fac Med Xavier Bichat Paris France 18 RM, U478, F-75870 Paris 18, France Fac Med Xavier Bichat, INSERM, U426, F-75870 Paris, France Fac Med Xavier Bichat Paris France F-75870 , U426, F-75870 Paris, France CNRS, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France CNRS Valbonne France F-06560 Mol & Cellulaire, F-06560 Valbonne, France
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 44, anno: 1998,
pagine: C1602 - C1609
SICI:
0363-6143(199812)44:6<C1602:EOTANW>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CORTICAL COLLECTING TUBULE; K+ CHANNEL; APICAL MEMBRANE; GENETIC-HETEROGENEITY; BARTTERS-SYNDROME; MUTATIONS; CLONING; PROTEIN; ROMK; LOCALIZATION;
Keywords:
potassium secretion; collecting duct; loop of Henle; proximal tubule; aquaporin-2; Tamm-Horsfall protein; immunolocalization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Farman, N Fac Med Xavier Bichat, INSERM, U478, BP 416, F-75870 Paris 18, France Fac Med Xavier Bichat BP 416 Paris France 18 0 Paris 18, France
Citazione:
F. Cluzeaud et al., "Expression of TWIK-1, a novel weakly inward rectifying potassium channel in rat kidney", AM J P-CELL, 44(6), 1998, pp. C1602-C1609

Abstract

Several K+ conductances have been identified in the kidney, with specific properties and localization in distinct cell types and membrane domains. Onthe other hand, several K+ channels have been characterized at the molecular level. By immunolocalization, we show that a new inward rectifying K+ channel, TWIK-1, is specifically expressed in distinct tubular segments and cell types of the rat kidney. In the proximal tubule, TWIK-1 prevails in theinitial portions (convoluted part), where it is restricted to the apica (brush-border) membrane. In the collecting duct, immunofluorescence was intracellular or confined to the apical membrane and restricted to intercalated cells, i.e., in cells lacking aquaporin-2, as shown by double immunofluorescence. TWIK was also expressed in medullary and cortical parts of the thicklimb of the loop of Henle, identified with an anti-Tamm-Horsfall protein antibody (double immunofluorescence). The intensity of TWIK-1 immunolabelingwas unchanged in rats fed a low-Na+ or a low-K+ diet. Because TWIK-1 shares common properties with the low-conductance apical K+ channel of the collecting duct, we propose that it could play a role in K+ secretion, complementary to ROMK, another recently characterized K+ channel located in principal cells of the cortical collecting duct and in the loop of Henle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:24:45