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Titolo:
SORTING OF ACTIN ISOFORMS IN CHICKEN AUDITORY HAIR-CELLS
Autore:
HOFER D; NESS W; DRENCKHAHN D;
Indirizzi:
UNIV WURZBURG,INST ANAT,KOELLIKERSTR 6 D-97070 WURZBURG GERMANY UNIV WURZBURG,INST ANAT D-97070 WURZBURG GERMANY
Titolo Testata:
Journal of Cell Science
, volume: 110, anno: 1997,
parte:, 6
pagine: 765 - 770
SICI:
0021-9533(1997)110:<765:SOAIIC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-ACTIN; GAMMA-ACTIN; CUTICULAR PLATE; MESSENGER-RNAS; CYTOPLASMIC ACTIN; PLASMA-MEMBRANE; SKELETAL-MUSCLE; FILAMENTS; IDENTIFICATION; PROTEINS;
Keywords:
ACTIN ISOFORM; HAIR CELL; STEREOCILIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
D. Hofer et al., "SORTING OF ACTIN ISOFORMS IN CHICKEN AUDITORY HAIR-CELLS", Journal of Cell Science, 110, 1997, pp. 765-770

Abstract

Most nonmuscle cells of higher vertebrates contain two different actin isoforms, beta- and gamma-cytoplasmic actin. The beta-isoform is with few exceptions the predominant isoform in nonmuscle cells and tissues, Perturbation of the beta:gamma ratio has been shown to affect the organization of bundled actin filaments indicating that the beta- and gamma-genes encode functionally distinct cytoarchitectural information. In the present study we localized by immunostaining beta- and gamma-actin in chicken auditory hair cells, These highly specialized cells serve as model system for studying certain developmental and structural aspects of a complex actin filament system with high architectural precision, We show that gamma-actin is the predominant actin isoform in auditory hair cells with an apparent beta:gamma ratio of similar to 1:2. gamma-Actin is not sorted and occurs in all three actin assemblies of the hair border, i.e. the cores of sensory hairs (stereocilia), the subjacent gel-like actin filament meshwork (cuticular plate) and the zonula adherens ring, In contrast to gamma-actin, the beta-isoform is specifically sorted to the actin filament core bundle of stereocilia that is extensively crosslinked by fimbrin. In view of recent studies showing that L-plastin, the leukocyte homolog of fimbrin, has a higher binding affinity for beta-actin than for gamma-actin. a mechanism is proposed for how hair cells might restrict formation of actin filament bundles to a single cellular site (i.e. the stereocilia). The limited level of expression of beta-actin in hair cells may help to prevent ectopic bundle formation in other cellular compartments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 15:41:44