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Titolo:
Miniature Ca2+ channels in excised plasma-membrane patches: activation by IP3
Autore:
Kiselyov, KI; Semyonova, SB; Mamin, AG; Mozhayeva, GN;
Indirizzi:
Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia Russian Acad Sci St Petersburg Russia 194064 t Petersburg 194064, Russia
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 2, volume: 437, anno: 1999,
pagine: 305 - 314
SICI:
0031-6768(199901)437:2<305:MCCIEP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; CAPACITATIVE CALCIUM-ENTRY; PANCREATIC ACINAR-CELLS; ENDOPLASMIC-RETICULUM; ARACHIDONIC-ACID; CARCINOMA-CELLS; MAST-CELLS; T-CELLS; RELEASE; STORES;
Keywords:
Ca2+ channels; IP3; Ca2+-release-activated Ca2+ channels; excised plasma-membrane patches; IP3 receptors; mouse macrophages; A431 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Mozhayeva, GN Russianiacad Sci, Inst Cytol, Tikhoretsky Ave 4, St Petersburg 194064, Russ Russian Acad Sci Tikhoretsky Ave 4 St Petersburg Russia 194064
Citazione:
K.I. Kiselyov et al., "Miniature Ca2+ channels in excised plasma-membrane patches: activation by IP3", PFLUG ARCH, 437(2), 1999, pp. 305-314

Abstract

In the present work, we characterized the receptor properties and the conductive features of the inositol (1,4,5)-trisphosphate (IP3)-activated Ca2+ channels present in excised plasma-membrane patches obtained from mouse macrophages and A431 cells. We found that the receptor properties of the channels tested were similar to those of the IP3 receptor (IP3R) expressed in the endoplasmic reticulum (ER) membrane. These properties include activation by IP3, inhibition by heparin, time-dependent inactivation by high IP3 concentrations, activation by guanosine 5'o-thiotriphosphate and regulation by arachidonic acid. On the other hand, in terms of conductive properties, thechannel closely resembles Ca2+-release-activated Ca2+ channels (I-crac). These conductive properties include extremely low conductance (approximate to 1 pS), very high selectivity for Ca2+ over K+ (P-Ca/P-K > 1000), inactivation by high intracellular Ca2+ concentration and, importantly, strong inward rectification. Notably, the same channel was activated by: (1) agonists in the cell-attached mode of channel recording, and (2) cytosolic IP3 afterpatch excision. Although the possibility cannot be completely excluded that a novel type of IP3R is expressed exclusively in the plasma membrane, in their entirety our findings suggest that the plasma membrane of mouse macrophages and A431 cells contains I-crac-like Ca2+ channels coupled to an IP3-responsive protein which displays properties similar to those of the IP3R expressed in the ER membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 01:38:51