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Titolo:
Spontaneous Na+ and Ca2+ spike firing of cerebellar Purkinje neurons at high pressure
Autore:
Etzion, Y; Grossman, Y;
Indirizzi:
Benaelrion Univ Negev, Fac Hlth Sci, Dept Physiol, IL-84105 Beer Sheva, Isr Ben Gurion Univ Negev Beer Sheva Israel IL-84105 L-84105 Beer Sheva, Isr BenSheva,n Univ Negev, Fac Hlth Sci, Zlotowski Ctr Neurosci, IL-84105 BeerBen Gurion Univ Negev Beer Sheva Israel IL-84105 Neurosci, IL-84105 Beer
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 2, volume: 437, anno: 1999,
pagine: 276 - 284
SICI:
0031-6768(199901)437:2<276:SNACSF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM CHANNELS; HYDROSTATIC-PRESSURE; IN-VITRO; NEUROLOGICAL SYNDROME; INHIBITORY SYNAPSES; MAMMALIAN NEURONS; HELIUM PRESSURE; CELLS; DENDRITES; RESPONSES;
Keywords:
cerebellum; dendrite physiology; K+ channel; macropatch clamp; rhythmic firing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Etzion, Y Benheva,on Univ Negev, Fac Hlth Sci, Dept Physiol, POB 653, IL-84105 Beer S Ben Gurion Univ Negev POB 653 Beer Sheva Israel IL-84105 Beer S
Citazione:
Y. Etzion e Y. Grossman, "Spontaneous Na+ and Ca2+ spike firing of cerebellar Purkinje neurons at high pressure", PFLUG ARCH, 437(2), 1999, pp. 276-284

Abstract

The effects of high pressure (up to 10.1 MPa) on the spontaneous firing ofPurkinje neurons in guineapig cerebellar slices were studied using the macropatch clamp technique. Pressure did not significantly alter the single somatic Naf spike parameters or the frequency of regular Na+ spike firing. When Na+ currents were blocked by 0.5-1 mu M tetrodotoxin (TTX), a pressure of 10.1 MPa slightly reduced the dendritic Ca2+ spike amplitude to 90.2 +/- 3.1% of its control value, and slowed its kinetics. The effects of pressureon the single Ca2+ spike were even less prominent when Kf currents were blocked by 5 mM 4-aminopyridine (4-AP). Pressure prolonged the active period of Ca2+ spike firing to 152.2 +/- 10.4% of the control value. Within the active period pressure increased the inter-spike interval to 164.9 +/- 8.7% and suppressed the typical firing of doublets. The latter changes were reversed by a high extracellular potassium concentration ([K+](o)) and 1 mu M 4-AP, whereas in the presence of 5 mM 4-AP the pattern was insensitive to pressure. A high [Ca2+], reduced the firing frequency and suppressed doublet firing in a manner reminiscent of the pressure effect, but these changes could not be reversed by 4-AP A low [Ca2+], slightly increased the firing of doublets. These results show that the single somatic Na+ spike is insensitive and the dendritic Ca2+ spike is only mildly sensitive to pressure. However, alterations in Ca2+ spike firing pattern suggest that modulation of dendritic Kf currents induce depression of dendritic excitability at pressure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:02:20