Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Localization of a pathogenicity gene in Ophiostoma novo-ulmi and evidence that it may be introgressed from O-ulmi
Autore:
Et-Touil, A; Brasier, CM; Bernier, L;
Indirizzi:
Univ Laval, Ctr Rech Biol Forestiere, St Foy, PQ G1K 7P4, Canada Univ Laval St Foy PQ Canada G1K 7P4 orestiere, St Foy, PQ G1K 7P4, Canada Forest Res Stn, Farnham GU10 4LH, Surrey, England Forest Res Stn Farnham Surrey England GU10 4LH GU10 4LH, Surrey, England
Titolo Testata:
MOLECULAR PLANT-MICROBE INTERACTIONS
fascicolo: 1, volume: 12, anno: 1999,
pagine: 6 - 15
SICI:
0894-0282(199901)12:1<6:LOAPGI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUTCH ELM DISEASE; BULKED SEGREGANT ANALYSIS; NEAR-ISOGENIC LINES; CERATO-ULMIN; MOLECULAR RELATIONSHIPS; LENGTH-POLYMORPHISMS; RAPD MARKERS; EAN RACES; IDENTIFICATION; INHERITANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Bernier, L Univ7P4,al, Ctr Rech Biol Forestiere, Pavillon CE Marchand, St Foy, PQ G1K Univ Laval Pavillon CE Marchand St Foy PQ Canada G1K 7P4 PQ G1K
Citazione:
A. Et-Touil et al., "Localization of a pathogenicity gene in Ophiostoma novo-ulmi and evidence that it may be introgressed from O-ulmi", MOL PL MICR, 12(1), 1999, pp. 6-15

Abstract

Ophiostoma novo-ulmi, the principal agent of Dutch elm disease, has recently replaced another species of Dutch elm disease pathogen, O. ulmi, across much of the Northern Hemisphere, Field inoculations of the moderately resistant elms Ulmus procera and Ulmus x Commelin were carried out with progeny of a genetic cross between AST27, a Eurasian (EAN) O, novo-ulmi isolate with an unusually low level of pathogenicity, and H327, a highly aggressive EAN isolate. These confirmed the results of a previous study that indicated that the difference in phenotype was controlled by a single nuclear gene. This pathogenicity gene, designated here Pat1, is the first putative pathogenicity gene to be identified in O, novo-ulmi. In a bulked segregant analysis, involving 80 random primers, 10 RAPD (random amplified polymorphic DNA) markers were identified linked to Pat1, Linkage distances between these markers and Pat1 were confirmed by genetic analysis of all individual progeny. Five RAPD amplicons identified in AST27 were O. ulmi and not O. novo-ulmi specific amplicons, and of these two were linked to Pat1, This suggests thatthe Pat1 allele conferring unusually low aggressiveness in AST27 may have been acquired from O, ulmi via introgression, When RAPD marker OPK3(1050), linked to Pat1, was used as a probe for Southern hybridization with electrophoretically separated chromosomes of O, novo-ulmi and O, ulmi, the resultsindicated that Pat1 is located on a 3.5 Mb chromosome previously designated chDNA II.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:48:22