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Titolo:
A new method for the rapid and long term growth of human neural precursor cells
Autore:
Svendsen, CN; ter Borg, MG; Armstrong, RJE; Rosser, AE; Chandran, S; Ostenfeld, T; Caldwell, MA;
Indirizzi:
Cambridge2PY,v Forvie Site, MRC, Cambridge Ctr Brain Repair, Cambridge CB2Cambridge Univ Forvie Site Cambridge England CB2 2PY pair, Cambridge CB2
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 2, volume: 85, anno: 1998,
pagine: 141 - 152
SICI:
0165-0270(199812)85:2<141:ANMFTR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; HUMAN FETAL BRAIN; STEM-CELLS; PROGENITOR CELLS; IN-VITRO; CNS PRECURSOR; RAT-BRAIN; EGF; SURVIVAL; MOUSE;
Keywords:
stem cell; progenitor cell; proliferation; differentiation; expansion; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Svendsen, CN CambridgeCambridgevie Site, MRC, Cambridge Ctr Brain Repair, Robinson Way, Cambridge Univ Forvie Site Robinson Way Cambridge England CB2 2PY
Citazione:
C.N. Svendsen et al., "A new method for the rapid and long term growth of human neural precursor cells", J NEUROSC M, 85(2), 1998, pp. 141-152

Abstract

A reliable source of human neural tissue would be of immense practical value to both neuroscientists and clinical neural transplantation trials. In this study, human precursor cells were isolated from the developing human cortex and, in the presence of both epidermal and fibroblast growth factor-2,grew in culture as sphere shaped clusters. Using traditional passaging techniques and culture mediums the rate of growth was extremely slow, and onlya 12-fold expansion in total cell number could be achieved. However, when intact spheres were sectioned into quarters, rather than mechanically dissociated, cell-cell contacts were maintained and cellular trauma minimised which permitted the rapid and continual growth of each individual quarter. Using this method we have achieved a 1.5 million-fold increase in precursor cell number over a period of less than 200 days. Upon differentiation by exposure to a substrate, cells migrated out from the spheres and formed a monolayer of astrocytes and neurons. No oligodendrocytes were found to develop from these human neural precursor cells at late passages when whole sphereswere differentiated. This simple and novel culture method allows the rapidexpansion of large numbers of non-transformed human neural precursor cellswhich may be of use in drug discovery, ex vivo gene therapy and clinical neural transplantation. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:15:15